Cell Biology Antibodies 6
Anti-DPYD Antibody (CAB1620)
- SKU:
- CAB1620
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DPYD Antibody |
| Antibody SKU: | CAB1620 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human DPYD (NP_001153773.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | THP-1, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human DPYD (NP_001153773.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPV LSKD SADI ESIL ALNP RTQT HATL CSTS AKKL DKKH WKRN PDKN CFNC EKLE NNFD DIKH TTLG ERGA LREA MRCL KCAD APCQ KSCP TNLD IKSF ITSI ANKN YYGA AKMI FSDN PLGL TCGM VCPT SDLC VGGC NLYA TEEG PINI GGLQ QFAT ETLI LAFS LMNH L |
| Gene ID: | 1806 |
| Uniprot: | Q12882 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 18kDa/111kDa |
| Observed MW: | 112kDa |
| Synonyms: | DPYD, DHP, DHPDHASE, DPD |
| Background: | The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - beta-alanine; EC 1.3.1.2; Xenobiotic Metabolism - drug metabolism - other enzymes; Oxidoreductase; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis Chromosomal Location of Human Ortholog: 1p22 Cellular Component: cytoplasm; cytosol Molecular Function:protein homodimerization activity; FAD binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; UMP biosynthetic process; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; pyrimidine base catabolic process; thymidine catabolic process Disease: Dihydropyrimidine Dehydrogenase Deficiency |
| NCBI Summary: | The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | Q12882 |
| NCBI GenInfo Identifier: | 160332325 |
| NCBI Gene ID: | 1806 |
| NCBI Accession: | Q12882.2 |
| UniProt Secondary Accession: | Q12882,Q16694, Q16761, Q32NB0, Q96HL6, Q96TH1, A2RRQ2 A2RRQ3, A8K5A2, A8MWG9, B1AN21, E9PFN1, |
| UniProt Related Accession: | Q12882 |
| Molecular Weight: | 18,936 Da |
| NCBI Full Name: | Dihydropyrimidine dehydrogenase |
| NCBI Synonym Full Names: | dihydropyrimidine dehydrogenase |
| NCBI Official Symbol: | DPYD |
| NCBI Official Synonym Symbols: | DHP; DPD; DHPDHASE |
| NCBI Protein Information: | dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase |
| UniProt Protein Name: | Dihydropyrimidine dehydrogenase [NADP(+)] |
| UniProt Synonym Protein Names: | Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase |
| UniProt Gene Name: | DPYD |
| UniProt Entry Name: | DPYD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DPYD Antibody (CAB1620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
Related Products
