Epigenetics & Nuclear Signaling Antibodies 1

Anti-DPPA4 Antibody (CAB13724)

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SKU:
CAB13724
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DPPA4 Antibody
Antibody SKU:CAB13724
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 15-304 of human DPPA4 (NP_060659.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 15-304 of human DPPA4 (NP_060659.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KGKE WTST EKSR EEDQ QASN QPNS IALP GTSA KRTK EKMS IKGS KVLC PKKK AEHT DNPR PQKK IPIP PLPS KLPP VNLI HRDI LRAW CQQL KLSS KGQK LDAY KRLC AFAY PNQK DFPS TAKE AKIR KSLQ KKLK VEKG ETSL QSSE THPP EVAL PPVG EPPA LENS TALL EGVN TVVV TTSA PEAL LASW ARIS ARAR TPEA VESP QEAS GVRW CVVH GKSL PADT DGWV HLQF HAGQ AWVP EKQE GRVS ALFL LPAS NFPP PHLE DNML CPKC VHRN KVLI KSLQ WE
Gene ID:55211
Uniprot:Q7L190
Cellular Location:Nucleus
Calculated MW:33kDa
Observed MW:38kDa
Synonyms:DPPA4, 2410091M23Rik
Background:This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:DPPA4: May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
UniProt Protein Details:

Protein type:Cell development/differentiation

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: nucleoplasm

Molecular Function:protein binding

Biological Process: somatic stem cell maintenance

UniProt Code:Q7L190
NCBI GenInfo Identifier:296434484
NCBI Gene ID:55211
NCBI Accession:Q7L190.2
UniProt Secondary Accession:Q7L190,Q9H9N5, Q9NVI6, A8K4M7,
UniProt Related Accession:Q7L190
Molecular Weight:33,541 Da
NCBI Full Name:Developmental pluripotency-associated protein 4
NCBI Synonym Full Names:developmental pluripotency associated 4
NCBI Official Symbol:DPPA4  
NCBI Official Synonym Symbols:2410091M23Rik  
NCBI Protein Information:developmental pluripotency-associated protein 4
UniProt Protein Name:Developmental pluripotency-associated protein 4
Protein Family:Developmental pluripotency-associated protein
UniProt Gene Name:DPPA4  
UniProt Entry Name:DPPA4_HUMAN
Anti-DPPA4 Antibody (CAB13724)Western blot analysis of extracts of various cell lines, using DPPA4 antibody (CAB13724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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