Neuroscience

Anti-DPF3 Antibody (CAB16890)

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SKU:
CAB16890
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-DPF3 Antibody
Antibody SKU:CAB16890
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-200 of human DPF3 (NP_036206.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:mouse heart, mouse brain, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-200 of human DPF3 (NP_036206.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DPKL RLLE IKPE VELP LKKD GFTS ESTT LEAL LRGE GVEK KVDA REEE SIQE IQRV LEND ENVE EGNE EEDL EEDI PKRK NRTR GRAR GSAG GRRR HDAA SQED HDKP YVC
Gene ID:8110
Uniprot:Q92784
Cellular Location:
Calculated MW:43kDa
Observed MW:43kDa
Synonyms:DPF3, BAF45C, CERD4
Background:This gene encodes a member of the D4 protein family. The encoded protein is a transcription regulator that binds acetylated histones and is a component of the BAF chromatin remodeling complex. Alternate splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:DPF3: Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. Belongs to the requiem/DPF family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 14q24.2

Molecular Function:zinc ion binding

Biological Process: nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification

NCBI Summary:This gene encodes a member of the D4 protein family. The encoded protein is a transcription regulator that binds acetylated histones and is a component of the BAF chromatin remodeling complex. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:Q92784
NCBI GenInfo Identifier:526118057
NCBI Gene ID:8110
NCBI Accession:NP_001267471.1
UniProt Secondary Accession:Q92784,Q32UJ0, Q6P9E6, Q6ZT41, Q9H7Y5, A8MSI3, B7Z276 F5H575,
UniProt Related Accession:Q92784
Molecular Weight:
NCBI Full Name:zinc finger protein DPF3 isoform 2
NCBI Synonym Full Names:D4, zinc and double PHD fingers, family 3
NCBI Official Symbol:DPF3  
NCBI Official Synonym Symbols:CERD4; BAF45C  
NCBI Protein Information:zinc finger protein DPF3
UniProt Protein Name:Zinc finger protein DPF3
UniProt Synonym Protein Names:BRG1-associated factor 45C; BAF45C; Zinc finger protein cer-d4
Protein Family:Zinc finger protein
UniProt Gene Name:DPF3  
UniProt Entry Name:DPF3_HUMAN
Anti-DPF3 Antibody (CAB16890)Western blot analysis of extracts of various cell lines, using DPF3 antibody (CAB16890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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