Cell Biology Antibodies 11
Anti-Dot1L/KMT4 Antibody (CAB7619)
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- SKU:
- CAB7619
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Dot1L/KMT4 Antibody |
| Antibody SKU: | CAB7619 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4/KMT4 (NP_115871.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4/KMT4 (NP_115871.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DHQL KERF ANMK EGGR IVSS KPFA PLNF RINS RNLS DIGT IMRV VELS PLKG SVSW TGKP VSYY LHTI DRTI LENY FSSL KNPK LREE QEAA RRRQ QRES KSNA ATPT KGPE GKVA GPAD APMD SGAE EEKA GAAT VKKP SPSK ARKK KLNK KGRK MAGR KRGR PKKM NTAN PERK PKKN QTAL DALH AQTV SQTA ASSP QDAY RSPH SPFY QLPP SVQR HSPN PLLV APTP PALQ KLLE SFKI QYLQ FLA |
| Gene ID: | 84444 |
| Uniprot: | Q8TEK3 |
| Cellular Location: | Nucleus |
| Calculated MW: | 164kDa/184kDa |
| Observed MW: | 160kDa |
| Synonyms: | DOT1L, DOT1, KMT4 |
| Background: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. |
| UniProt Protein Function: | DOT1L: a protein-lysine N-methyltransferase of the seven beta-strand class. Catalyzes the mono-, di- and tri-methylation of H3K79. Methylated H3K79 is associated with active gene transcription. DOT1L activity drives proliferation of mixed lineage leukemia 1 protein (MLL)-rearranged leukemias, inducing the aberrant transcription of a number of leukemogenic genes including HOXA9 and MEIS1. Binds to DNA. Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex Molecular Function:histone lysine N-methyltransferase activity (H3-K79 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding Biological Process: chromatin silencing at telomere; DNA damage checkpoint; DNA repair; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of JAK-STAT cascade; telomere organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q8TEK3 |
| NCBI GenInfo Identifier: | 25090171 |
| NCBI Gene ID: | 84444 |
| NCBI Accession: | Q8TEK3.2 |
| UniProt Secondary Accession: | Q8TEK3,O60379, Q96JL1, |
| UniProt Related Accession: | Q8TEK3 |
| Molecular Weight: | 185kDa |
| NCBI Full Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| NCBI Synonym Full Names: | DOT1 like histone lysine methyltransferase |
| NCBI Official Symbol: | DOT1L |
| NCBI Official Synonym Symbols: | DOT1; KMT4 |
| NCBI Protein Information: | histone-lysine N-methyltransferase, H3 lysine-79 specific |
| UniProt Protein Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| UniProt Synonym Protein Names: | DOT1-like protein; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4 |
| UniProt Gene Name: | DOT1L |
 | Western blot analysis of extracts of HeLa cells, using Dot1L/KMT4 antibody (CAB7619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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