Cell Biology Antibodies 2
Anti-Dot1L/KMT4 Antibody (CAB12328)
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- SKU:
- CAB12328
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Dot1L/KMT4 Antibody |
| Antibody SKU: | CAB12328 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human Dot1L/KMT4 (NP_115871.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human Dot1L/KMT4 (NP_115871.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TDKT PLLS GKAA KARD REVD LKNG HNLF ISAA AVPP GSLL SGPG LAPA ASSA GGAA SSAQ THRS FLGP FPPG PQFA LGPM SLQA NLGS VAGS SVLQ SLFS S |
| Gene ID: | 84444 |
| Uniprot: | Q8TEK3 |
| Cellular Location: | Nucleus |
| Calculated MW: | 164kDa/184kDa |
| Observed MW: | 170kDa |
| Synonyms: | DOT1L, DOT1, KMT4 |
| Background: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. |
| UniProt Protein Function: | DOT1L: a protein-lysine N-methyltransferase of the seven beta-strand class. Catalyzes the mono-, di- and tri-methylation of H3K79. Methylated H3K79 is associated with active gene transcription. DOT1L activity drives proliferation of mixed lineage leukemia 1 protein (MLL)-rearranged leukemias, inducing the aberrant transcription of a number of leukemogenic genes including HOXA9 and MEIS1. Binds to DNA. Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex Molecular Function:histone lysine N-methyltransferase activity (H3-K79 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding Biological Process: chromatin silencing at telomere; DNA damage checkpoint; DNA repair; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of JAK-STAT cascade; telomere organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q8TEK3 |
| NCBI GenInfo Identifier: | 25090171 |
| NCBI Gene ID: | 84444 |
| NCBI Accession: | Q8TEK3.2 |
| UniProt Secondary Accession: | Q8TEK3,O60379, Q96JL1, |
| UniProt Related Accession: | Q8TEK3 |
| Molecular Weight: | 185kDa |
| NCBI Full Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| NCBI Synonym Full Names: | DOT1 like histone lysine methyltransferase |
| NCBI Official Symbol: | DOT1L |
| NCBI Official Synonym Symbols: | DOT1; KMT4 |
| NCBI Protein Information: | histone-lysine N-methyltransferase, H3 lysine-79 specific |
| UniProt Protein Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| UniProt Synonym Protein Names: | DOT1-like protein; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4 |
| UniProt Gene Name: | DOT1L |
 | Western blot analysis of extracts of various cell lines, using Dot1L/KMT4 antibody (CAB12328) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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