Name: Anti-DOK5 Antibody (CAB13726)
Brand: Cell Biology Antibodies 4
SKU: CAB13726
Availability: OutOfStock

Antibody Name:	Anti-DOK5 Antibody
Antibody SKU:	CAB13726
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-306 of human DOK5 (NP_060901.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Mouse liver, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-306 of human DOK5 (NP_060901.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLQS VKNS MLQM KMSE RAAS LSTM VPLP RSAY WQHI TRQH STGQ LYRL QDVS SPLK LHRT ETFP AYRS EH
Gene ID:	55816
Uniprot:	Q9P104
Cellular Location:
Calculated MW:	22kDa/35kDa
Observed MW:	30kDa

Synonyms:

DOK5, C20orf180, IRS-6, IRS6

Background:

The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	DOK5: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. Interacts with phosphorylated Ret. In contrast to other DOK proteins, it does not interact with RASGAP. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: intracellular Molecular Function:insulin receptor binding; receptor signaling protein activity Biological Process: MAPKKK cascade; nervous system development; regulation of nerve growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Code:	Q9P104
NCBI GenInfo Identifier:	26393190
NCBI Gene ID:	55816
NCBI Accession:	Q9P104.2
UniProt Secondary Accession:	Q9P104,Q5T7Y0, Q5TE53, Q8TEW7, Q96H13, Q9BZ24, Q9NQF4 Q9Y411,
UniProt Related Accession:	Q9P104
Molecular Weight:	22,824 Da
NCBI Full Name:	Docking protein 5
NCBI Synonym Full Names:	docking protein 5
NCBI Official Symbol:	DOK5
NCBI Official Synonym Symbols:	IRS6; IRS-6; C20orf180
NCBI Protein Information:	docking protein 5
UniProt Protein Name:	Docking protein 5
UniProt Synonym Protein Names:	Downstream of tyrosine kinase 5; Insulin receptor substrate 6; IRS-6; IRS6
Protein Family:	Docking protein
UniProt Gene Name:	DOK5
UniProt Entry Name:	DOK5_HUMAN

Western blot analysis of extracts of various cell lines, using DOK5 antibody (CAB13726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

DOK5 Antibody (PACO03007)

DOK5 Antibody (PACO08916)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)