Cell Biology Antibodies 1

Anti-DOK4 Antibody (CAB0226)

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SKU:
CAB0226
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DOK4 Antibody
Antibody SKU:CAB0226
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-326 of human DOK4 (NP_060580.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-326 of human DOK4 (NP_060580.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EMEK NVRL LNKG TEHY SYPC TPTT MLPR SAYW HHIT GSQN IAEA SSYA GEGY GAAQ ASSE TDLL NRFI LLKP KPSQ GDSS EAKT PSQ
Gene ID:55715
Uniprot:Q8TEW6
Cellular Location:
Calculated MW:37kDa
Observed MW:37kDa
Synonyms:DOK4, IRS-5, IRS5
Background:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.
UniProt Protein Function:DOK4: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. Interacts with Ret and TIE2. Interaction with Ret is mediated through the PTB domain and requires phosphorylation of Ret Y1062.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Cell development/differentiation

Chromosomal Location of Human Ortholog: 16q21

Molecular Function:protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: nervous system development; MAPKKK cascade; transmembrane receptor protein tyrosine kinase signaling pathway

UniProt Code:Q8TEW6
NCBI GenInfo Identifier:84029600
NCBI Gene ID:55715
NCBI Accession:Q8TEW6.2
UniProt Secondary Accession:Q8TEW6,O75209, Q9BTP2, Q9NVV3,
UniProt Related Accession:Q8TEW6
Molecular Weight:326
NCBI Full Name:Docking protein 4
NCBI Synonym Full Names:docking protein 4
NCBI Official Symbol:DOK4  
NCBI Protein Information:docking protein 4; IRS5; IRS-5; insulin receptor substrate 5; downstream of tyrosine kinase 4
UniProt Protein Name:Docking protein 4
UniProt Synonym Protein Names:Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5
Protein Family:Docking protein
UniProt Gene Name:DOK4  
UniProt Entry Name:DOK4_HUMAN
Anti-DOK4 Antibody (CAB0226)Western blot analysis of extracts of MCF-7 cells, using DOK4 antibody (CAB0226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-DOK4 Antibody (CAB0226)Immunohistochemistry of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (CAB0226) at dilution of 1:100 (40x lens).
Anti-DOK4 Antibody (CAB0226)Immunofluorescence analysis of HeLa cells using DOK4 antibody (CAB0226). Blue: DAPI for nuclear staining.
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