Signal Transduction Antibodies 2

Anti-DOCK4 Antibody (CAB15764)

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SKU:
CAB15764
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DOCK4 Antibody
Antibody SKU:CAB15764
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 150-450 of human DOCK4 (NP_055520.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:DU145
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 150-450 of human DOCK4 (NP_055520.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LDWG NEQL GLDL VPRK EYAM VDPE DISI TELY RLME HRHR KKDT PVQA SSHH LFVQ MKSL MCSN LGEE LEVI FSLF DSKE NRPI SERF FLRL NRNG LPKA PDKP ERHC SLFV DLGS SELR KDIY ITVH IIRI GRMG AGEK KNAC SVQY RRPF GCAV LSIA DLLT GETK DDLI LKVY MCNT ESEW YQIH ENII KKLN ARYN LTGS NAGL AVSL QLLH GDIE QIRR EYSS VFSH GVSI TRKL GFSN IIMP GEMR NDLY ITIE RGEF EKGG KSVA RNVE VTMF IVDS SGQT LKDF ISFG SGEP P
Gene ID:9732
Uniprot:Q8N1I0
Cellular Location:Cytoplasm, Endomembrane system, Peripheral membrane protein, cytosol
Calculated MW:29kDa/222kDa/225kDa/226kDa
Observed MW:235kDa
Synonyms:DOCK4
Background:This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized.
UniProt Protein Function:Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.
NCBI Summary:This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q8N1I0
NCBI GenInfo Identifier:92091572
NCBI Gene ID:9732
NCBI Accession:NP_055520
UniProt Related Accession:Q8N1I0
Molecular Weight:
NCBI Full Name:dedicator of cytokinesis protein 4 isoform 1
NCBI Synonym Full Names:dedicator of cytokinesis 4
NCBI Official Symbol:DOCK4  
NCBI Protein Information:dedicator of cytokinesis protein 4
UniProt Protein Name:Dedicator of cytokinesis protein 4
Protein Family:Dedicator of cytokinesis protein
UniProt Gene Name:DOCK4  
Anti-DOCK4 Antibody (CAB15764)Western blot analysis of extracts of DU145 cells, using DOCK4 antibody (CAB15764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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