Antibody Name:	Anti-DNMT3B Antibody
Antibody SKU:	CAB11079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DNMT3B (NP_008823.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, HCT116, A-549, Mouse testis, C6

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DNMT3B (NP_008823.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKGD TRHL NGEE DAGG REDS ILVN GACS DQSS DSPP ILEA IRTP EIRG RRSS SRLS KREV SSLL SYTQ DLTG DGDG EDGD GSDT PVMP KLFR ETRT RSES PAVR TRNN NSVS SRER HRPS PRST RGRQ GRNH VDES PVEF PATR SLRR RATA SAGT PWPS PPSS YLTI DLTD DTED THGT PQSS STPY ARLA QDSQ QGGM
Gene ID:	1789
Uniprot:	Q9UBC3
Cellular Location:	Nucleus
Calculated MW:	77-95kDa
Observed MW:	95kDa

Synonyms:

DNMT3B, ICF, ICF1, M.HsaIIIB

Background:

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

UniProt Protein Function:	Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.
NCBI Summary:	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]
UniProt Code:	Q9UBC3
NCBI GenInfo Identifier:	17375667
NCBI Gene ID:	1789
NCBI Accession:	Q9UBC3.1
UniProt Secondary Accession:	Q9UBC3,Q9UBD4, Q9UJQ5, Q9UKA6, Q9UNE5, A2A2E2, B4DSM8 B4DSU1, E1P5M6, E1P5M7, E7EN63, E9PBF2,
UniProt Related Accession:	Q9UBC3
Molecular Weight:	81,311 Da
NCBI Full Name:	DNA (cytosine-5)-methyltransferase 3B
NCBI Synonym Full Names:	DNA methyltransferase 3 beta
NCBI Official Symbol:	DNMT3B
NCBI Official Synonym Symbols:	ICF; ICF1; M.HsaIIIB
NCBI Protein Information:	DNA (cytosine-5)-methyltransferase 3B
UniProt Protein Name:	DNA (cytosine-5)-methyltransferase 3B
UniProt Synonym Protein Names:	DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIB
Protein Family:	DNA
UniProt Gene Name:	DNMT3B
UniProt Entry Name:	DNM3B_HUMAN

	Western blot analysis of extracts of various cell lines, using DNMT3B antibody (CAB11079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse liver using DNMT3B Rabbit pAb (CAB11079) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using DNMT3B Rabbit pAb (CAB11079) at dilution of 1:100 (40x lens).