Antibody Name:	Anti-Dnmt3a Antibody [KO Validated]
Antibody SKU:	CAB19659
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Dnmt3a.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, Mouse thymus, Mouse testis, Rat testis, 293T

Immunogen:	A synthesized peptide derived from human Dnmt3a.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1788
Uniprot:	Q9Y6K1
Cellular Location:
Calculated MW:	130kDa
Observed MW:	80KDa/140KDa

	Western blot analysis of extracts from normal (control) and Dnmt3a knockout (KO) 293T cells, using Dnmt3a antibody (CAB19659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
	Western blot analysis of extracts of various cell lines, using Dnmt3a antibody (CAB19659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.

Synonyms:

DNMT3A, DNMT3A2, M.HsaIIIA, TBRS, DNMT3A

Background:

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]

UniProt Protein Function:	DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
UniProt Protein Details:	Protein type:EC 2.1.1.37; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase Chromosomal Location of Human Ortholog: 2p23 Cellular Component: cytoplasm; euchromatin; nuclear matrix; nucleoplasm; nucleus Molecular Function:DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; identical protein binding; protein binding Biological Process: DNA methylation; genetic imprinting; negative regulation of gene expression, epigenetic Disease: Tatton-brown-rahman Syndrome
NCBI Summary:	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]
UniProt Code:	Q9Y6K1
NCBI GenInfo Identifier:	166215081
NCBI Gene ID:	1788
NCBI Accession:	Q9Y6K1.4
UniProt Secondary Accession:	Q9Y6K1,Q86TE8, Q86XF5, Q8IZV0, Q8WXU9, E9PEB8,
UniProt Related Accession:	Q9Y6K1
Molecular Weight:	16,866 Da
NCBI Full Name:	DNA (cytosine-5)-methyltransferase 3A
NCBI Synonym Full Names:	DNA methyltransferase 3 alpha
NCBI Official Symbol:	DNMT3A
NCBI Official Synonym Symbols:	TBRS; DNMT3A2; M.HsaIIIA
NCBI Protein Information:	DNA (cytosine-5)-methyltransferase 3A
UniProt Protein Name:	DNA (cytosine-5)-methyltransferase 3A
UniProt Synonym Protein Names:	DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA
Protein Family:	DNA
UniProt Gene Name:	DNMT3A
UniProt Entry Name:	DNM3A_HUMAN