KO Validated Antibodies 2

Anti-Dnmt1 Antibody [KO Validated] (CAB19679)

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SKU:
CAB19679
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Dnmt1 Antibody [KO Validated]
Antibody SKU:CAB19679
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Dnmt1.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, 293T
Immunogen:A synthesized peptide derived from human Dnmt1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1786
Uniprot:P26358
Cellular Location:
Calculated MW:183kDa
Observed MW:183KDa
Western blot - [KO Validated] DNMT1 Rabbit mAb Western blot analysis of extracts of various cell lines, using DNMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western blot - [KO Validated] DNMT1 Rabbit mAb Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - [KO Validated] DNMT1 Rabbit mAb Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] DNMT1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] DNMT1 Rabbit mAb Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] DNMT1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] DNMT1 Rabbit mAb Immunohistochemistry of paraffin-embedded mouse testis using [KO Validated] DNMT1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] DNMT1 Rabbit mAb Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] DNMT1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunofluorescence - [KO Validated] DNMT1 Rabbit mAb Immunofluorescence analysis of C6 cells using DNMT1 antibody (A19679). Blue: DAPI for nuclear staining.
Immunoprecipitation - [KO Validated] DNMT1 Rabbit mAb Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 antibody at a dilition of 1:1000.
Chromatin Immunoprecipitation - [KO Validated] DNMT1 Rabbit mAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DNMT1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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