Epigenetics & Nuclear Signaling Antibodies 5
Anti-DNMT1 Antibody (CAB17474)
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- SKU:
- CAB17474
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-DNMT1 Antibody |
| Antibody SKU: | CAB17474 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein of Human DNMT1. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, SW480 |
| Immunogen: | Recombinant fusion protein of Human DNMT1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1786 |
| Uniprot: | P26358 |
| Cellular Location: | Nucleus |
| Calculated MW: | 144kDa/183kDa/184kDa |
| Observed MW: | 200kDa |
 | Western blot analysis of extracts of Jurkat cells, using DNMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of Jurkat cells, using DNMT1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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