Cell Biology Antibodies 11

Anti-DNM2 Antibody (CAB7890)

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SKU:
CAB7890
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DNM2 Antibody
Antibody SKU:CAB7890
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 607-866 of human DNM2 (NP_004936.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 607-866 of human DNM2 (NP_004936.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EDVD SWKA SFLR AGVY PEKD QAEN EDGA QENT FSMD PQLE RQVE TIRN LVDS YVAI INKS IRDL MPKT IMHL MINN TKAF IHHE LLAY LYSS ADQS SLME ESAD QAQR RDDM LRMY HALK EALN IIGD ISTS TVST PVPP PVDD TWLQ SASS HSPT PQRR PVSS IHPP GRPP AVRG PTPG PPLI PVPV GAAA SFSA PPIP SRPG PQSV FANS DLFP APPQ IPSR PVRI PPGI PPGV PSRR PPAA PSRP TIIR PAEP SLLD
Gene ID:1785
Uniprot:P50570
Cellular Location:Cell junction, Cytoplasm, Membrane, Midbody, clathrin-coated pit, cytoskeleton, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:97kDa/98kDa
Observed MW:125kDa
Synonyms:DNM2, CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin-2
Background:Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
UniProt Protein Function:DYN2: a cytoplasmic microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. May mediate the conventional clathrin-mediated uptake of surface receptors. Expressed in the postsynaptic density of neuronal cells. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Microtubule-binding; EC 3.6.5.5; Motility/polarity/chemotaxis; Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi membrane; Golgi apparatus; postsynaptic membrane; microtubule; growth cone; focal adhesion; postsynaptic density; cytoplasm; plasma membrane; coated pit; midbody; cytosol

Molecular Function:GTPase activity; protein binding; enzyme binding; GTP binding; microtubule binding; SH3 domain binding

Biological Process: receptor-mediated endocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; synaptic vesicle transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; transferrin transport; endocytosis; regulation of axon extension; signal transduction; nitric oxide metabolic process; regulation of transcription, DNA-dependent; receptor internalization; post-Golgi vesicle-mediated transport; regulation of nitric-oxide synthase activity; G2/M transition of mitotic cell cycle; neurite morphogenesis

Disease: Lethal Congenital Contracture Syndrome 5; Myopathy, Centronuclear, 1; Charcot-marie-tooth Disease, Dominant Intermediate B

NCBI Summary:Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]
UniProt Code:P50570
NCBI GenInfo Identifier:47117856
NCBI Gene ID:1785
NCBI Accession:P50570.2
UniProt Secondary Accession:P50570,Q5I0Y0, Q7Z5S3, Q9UPH4, A8K1B6, E7EV30, E9PEQ4 K7ESI9,
UniProt Related Accession:P50570
Molecular Weight:870
NCBI Full Name:Dynamin-2
NCBI Synonym Full Names:dynamin 2
NCBI Official Symbol:DNM2  
NCBI Official Synonym Symbols:DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB  
NCBI Protein Information:dynamin-2; dynamin II
UniProt Protein Name:Dynamin-2
Protein Family:Dynamin
UniProt Gene Name:DNM2  
UniProt Entry Name:DYN2_HUMAN
Anti-DNM2 Antibody (CAB7890)Western blot analysis of extracts of various cell lines, using DNM2 Antibody (CAB7890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-DNM2 Antibody (CAB7890)Immunofluorescence analysis of NIH-3T3 cells using DNM2 Rabbit pAb (CAB7890) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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