Cell Biology Antibodies 3
Anti-DNASE1L1 Antibody (CAB13150)
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- SKU:
- CAB13150
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DNASE1L1 Antibody |
| Antibody SKU: | CAB13150 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHYP TALL FLIL ANGA QAFR ICAF NAQR LTLA KVAR EQVM DTLV RILA RCDI MVLQ EVVD SSGS AIPL LLRE LNRF DGSG PYST LSSP QLGR STYM ETYV YFYR SHKT QVLS SYVY NDED DVFA REPF VAQF SLPS NVLP SLVL VPLH TTPK AVEK ELNA LYDV FLEV SQHW QSKD VILL GDFN ADCA SLTK KRLD KLEL RTEP GFHW VIAD GEDT TVRA STHC TYDR VVLH GERC RSLL HTAA AFDF PTSF QLTE EEAL NISD HYPV EVEL KLSQ AHSV QPLS LTVL LLLS LLSP QLCP AA |
| Gene ID: | 1774 |
| Uniprot: | P49184 |
| Cellular Location: | Endoplasmic reticulum |
| Calculated MW: | 33kDa |
| Observed MW: | 34kDa |
| Synonyms: | DNASE1L1, DNAS1L1, DNASEX, DNL1L, G4.8, XIB |
| Background: | This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed. |
| UniProt Protein Function: | DNASE1L1: a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:EC 3.1.21.-; Deoxyribonuclease Chromosomal Location of Human Ortholog: Xq28 Cellular Component: endoplasmic reticulum; nucleus Molecular Function:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; DNA binding; deoxyribonuclease activity; endodeoxyribonuclease activity Biological Process: DNA catabolic process, endonucleolytic; DNA metabolic process |
| NCBI Summary: | This gene encodes a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49184 |
| NCBI GenInfo Identifier: | 1352319 |
| NCBI Gene ID: | 1774 |
| NCBI Accession: | P49184.1 |
| UniProt Secondary Accession: | P49184,Q5HY41, D3DWW7, |
| UniProt Related Accession: | P49184 |
| Molecular Weight: | 33,893 Da |
| NCBI Full Name: | Deoxyribonuclease-1-like 1 |
| NCBI Synonym Full Names: | deoxyribonuclease I-like 1 |
| NCBI Official Symbol: | DNASE1L1 |
| NCBI Official Synonym Symbols: | XIB; G4.8; DNL1L; DNASEX; DNAS1L1 |
| NCBI Protein Information: | deoxyribonuclease-1-like 1; DNase X; DNase I-like 1; DNase I-like, muscle-specific |
| UniProt Protein Name: | Deoxyribonuclease-1-like 1 |
| UniProt Synonym Protein Names: | DNase X; Deoxyribonuclease I-like 1; DNase I-like 1; Muscle-specific DNase I-like; XIB |
| UniProt Gene Name: | DNASE1L1 |
| UniProt Entry Name: | DNSL1_HUMAN |
 | Western blot analysis of extracts of mouse heart, using DNASE1L1 Antibody (CAB13150) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using DNASE1L1 antibody (CAB13150) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 antibody (CAB13150) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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