Cell Biology Antibodies 6
Anti-DNAJC24 Antibody (CAB15560)
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- SKU:
- CAB15560
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DNAJC24 Antibody |
| Antibody SKU: | CAB15560 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2-149 of human DNAJC24 (NP_859057.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse heart, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2-149 of human DNAJC24 (NP_859057.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAVE QMPK KDWY SILG ADPS ANIS DLKQ KYQK LILM YHPD KQST DVPA GTVE ECVQ KFIE IDQA WKIL GNEE TKRE YDLQ RCED DLRN VGPV DAQV YLEE MSWN EGDH SFYL SCRC GGKY SVSK DEAE EVSL ISCD TCSL IIEL LHYN |
| Gene ID: | 120526 |
| Uniprot: | Q6P3W2 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 9kDa/17kDa |
| Observed MW: | 17kDa |
| Synonyms: | DNAJC24, DPH4, JJJ3, ZCSL3 |
| Background: |
| UniProt Protein Function: | DNAJC24: Stimulates the ATPase activity of several Hsp70-type chaperones. Belongs to the DPH4 family. |
| UniProt Protein Details: | Protein type:Enzyme, misc.; Chaperone Chromosomal Location of Human Ortholog: 11p13 Molecular Function:ATPase activator activity; ferrous iron binding; zinc ion binding Biological Process: positive regulation of ATPase activity |
| NCBI Summary: | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q6P3W2 |
| NCBI GenInfo Identifier: | 66773991 |
| NCBI Gene ID: | 120526 |
| NCBI Accession: | Q6P3W2.1 |
| UniProt Secondary Accession: | Q6P3W2,A8K0V0, B1ALC1, I6L9B4, |
| UniProt Related Accession: | Q6P3W2 |
| Molecular Weight: | 9,693 Da |
| NCBI Full Name: | DnaJ homolog subfamily C member 24 |
| NCBI Synonym Full Names: | DnaJ heat shock protein family (Hsp40) member C24 |
| NCBI Official Symbol: | DNAJC24 |
| NCBI Official Synonym Symbols: | DPH4; JJJ3; ZCSL3 |
| NCBI Protein Information: | dnaJ homolog subfamily C member 24 |
| UniProt Protein Name: | DnaJ homolog subfamily C member 24 |
| UniProt Synonym Protein Names: | CSL-type zinc finger-containing protein 3; Diphthamide biosynthesis protein 4 |
| Protein Family: | DnaJ homolog subfamily |
| UniProt Gene Name: | DNAJC24 |
| UniProt Entry Name: | DJC24_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DNAJC24 antibody (CAB15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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