Antibody Name:	Anti-DNAJC19 Antibody
Antibody SKU:	CAB14961
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DNAJC19 (NP_660304.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-1080, Mouse pancreas, Mouse small intestine, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DNAJC19 (NP_660304.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAST VVAV GLTI AAAG FAGR YVLQ AMKH MEPQ VKQV FQSL PKSA FSGG YYRG GFEP KMTK REAA LILG VSPT ANKG KIRD AHRR IMLL NHPD KGGS PYIA AKIN EAKD LLEG QAKK
Gene ID:	131118
Uniprot:	Q96DA6
Cellular Location:	Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:	10kDa/12kDa
Observed MW:	12kDa

Synonyms:

DNAJC19, PAM18, TIM14, TIMM14

Background:

The protein encoded by this gene is thought to be part of a complex involved in the ATP-dependent transport of transit peptide-containing proteins from the inner cell membrane to the mitochondrial matrix. Defects in this gene are a cause of 3-methylglutaconic aciduria type 5 (MGA5), also known as dilated cardiomyopathy with ataxia (DCMA). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 2, 6, 10, 14 and 19.

UniProt Protein Function:	DNAJC19: Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity. Defects in DNAJC19 are the cause of 3-methylglutaconic aciduria type 5 (MGA5); also known as dilated cardiomyopathy with ataxia (DCMA). MGA5 is an autosomal recessive disorder characterized by early-onset dilated cardiomyopathy, growth failure, cerebellar ataxia causing significant motor delays, testicular dysgenesis, growth failure, and significant increases in urine organic acids, particularly 3-methylglutaconic acid and 3-methylglutaric acid. Belongs to the TIM14 family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Chaperone; Mitochondrial Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: protein complex; mitochondrion; mitochondrial inner membrane; integral to membrane Molecular Function:protein binding Biological Process: genitalia development; cellular protein metabolic process; protein folding; visual perception; protein targeting to mitochondrion Disease: 3-methylglutaconic Aciduria, Type V
NCBI Summary:	The protein encoded by this gene is thought to be part of a complex involved in the ATP-dependent transport of transit peptide-containing proteins from the inner cell membrane to the mitochondrial matrix. Defects in this gene are a cause of 3-methylglutaconic aciduria type 5 (MGA5), also known as dilated cardiomyopathy with ataxia (DCMA). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 2, 6, 10, 14 and 19. [provided by RefSeq, Jan 2012]
UniProt Code:	Q96DA6
NCBI GenInfo Identifier:	74760780
NCBI Gene ID:	131118
NCBI Accession:	Q96DA6.3
UniProt Secondary Accession:	Q96DA6,B2R4B1, C9JBV1,
UniProt Related Accession:	Q96DA6
Molecular Weight:	116
NCBI Full Name:	Mitochondrial import inner membrane translocase subunit TIM14
NCBI Synonym Full Names:	DnaJ (Hsp40) homolog, subfamily C, member 19
NCBI Official Symbol:	DNAJC19
NCBI Official Synonym Symbols:	PAM18; TIM14; TIMM14
NCBI Protein Information:	mitochondrial import inner membrane translocase subunit TIM14; homolog of yeast TIM14
UniProt Protein Name:	Mitochondrial import inner membrane translocase subunit TIM14
UniProt Synonym Protein Names:	DnaJ homolog subfamily C member 19
UniProt Gene Name:	DNAJC19
UniProt Entry Name:	TIM14_HUMAN

	Western blot analysis of extracts of various cell lines, using DNAJC19 Rabbit pAb (CAB14961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of Hela cells using DNAJC19 antibody (CAB14961) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.