Name: Anti-DNAJB4 Antibody (CAB13076)
Brand: Metabolism Antibodies 1
SKU: CAB13076
Availability: OutOfStock

Antibody Name:	Anti-DNAJB4 Antibody
Antibody SKU:	CAB13076
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human DNAJB4 (NP_008965.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human DNAJB4 (NP_008965.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EAYE VLSD PKKR EIYD QFGE EGLK GGAG GTDG QGGT FRYT FHGD PHAT FAAF FGGS NPFE IFFG RRMG GGRD SEEM EIDG DPFS AFGF SMNG YPRD RNSV GPSR LKQD PPV
Gene ID:	11080
Uniprot:	Q9UDY4
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	37kDa
Observed MW:	38kDa

Synonyms:

DNAJB4, DNAJW, DjB4, HLJ1

Background:

The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	DNAJB4: Probable chaperone. Homodimer. The C-terminal section interacts with the C- terminal tail of OPRM1. Interacts also with SDIM1. By heat shock. Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane Molecular Function:chaperone binding; protein binding; unfolded protein binding Biological Process: protein folding; response to heat; response to unfolded protein
NCBI Summary:	The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:	Q9UDY4
NCBI GenInfo Identifier:	8928155
NCBI Gene ID:	11080
NCBI Accession:	Q9UDY4.1
UniProt Secondary Accession:	Q9UDY4,Q13431, B2R824,
UniProt Related Accession:	Q9UDY4
Molecular Weight:	37,807 Da
NCBI Full Name:	DnaJ homolog subfamily B member 4
NCBI Synonym Full Names:	DnaJ heat shock protein family (Hsp40) member B4
NCBI Official Symbol:	DNAJB4
NCBI Official Synonym Symbols:	DjB4; HLJ1; DNAJW
NCBI Protein Information:	dnaJ homolog subfamily B member 4
UniProt Protein Name:	DnaJ homolog subfamily B member 4
UniProt Synonym Protein Names:	Heat shock 40 kDa protein 1 homolog; HSP40 homolog; Heat shock protein 40 homolog; Human liver DnaJ-like protein
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJB4
UniProt Entry Name:	DNJB4_HUMAN

Western blot analysis of extracts of various cell lines, using DNAJB4 antibody (CAB13076) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

DNAJB4 Antibody (PACO00716)

DNAJB4 Antibody (PACO08880)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)