Cell Biology Antibodies 1

Anti-DNAJB2 Antibody (CAB10345)

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SKU:
CAB10345
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DNAJB2 Antibody
Antibody SKU:CAB10345
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 215-324 of human DNAJB2 (NP_006727.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 215-324 of human DNAJB2 (NP_006727.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GLEL SRRE QQPS VTSR SGGT QVQQ TPAS CPLD SDLS EDED LQLA MAYS LSEM EAAG KKPA GGRE AQHR RQGR PKAQ HQDP GLGG TQEG ARGE ATKR SPSP EEKA SRCL IL
Gene ID:3300
Uniprot:P25686
Cellular Location:
Calculated MW:30kDa/35kDa
Observed MW:42kDa
Synonyms:DNAJB2, CMT2T, DSMA5, HSJ-1, HSJ1, HSPF3
Background:This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:DNAJB2: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Chaperone

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: proteasome complex; inclusion body; nucleus; cytosol

Molecular Function:protein binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: negative regulation of cell proliferation; ER-associated protein catabolic process; protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; negative regulation of cell growth; response to unfolded protein

Disease: Spinal Muscular Atrophy, Distal, Autosomal Recessive, 5; Charcot-marie-tooth Disease, Axonal, Type 2t

NCBI Summary:This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
UniProt Code:P25686
NCBI GenInfo Identifier:158518384
NCBI Gene ID:3300
NCBI Accession:P25686.3
UniProt Related Accession:P25686
Molecular Weight:Predicted Molecular Mass: 34.3kDaAccurate Molecular Mass: 37kDa
NCBI Full Name:DnaJ homolog subfamily B member 2
NCBI Synonym Full Names:DnaJ heat shock protein family (Hsp40) member B2
NCBI Official Symbol:DNAJB2  
NCBI Official Synonym Symbols:HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3  
NCBI Protein Information:dnaJ homolog subfamily B member 2
UniProt Protein Name:DnaJ homolog subfamily B member 2
UniProt Synonym Protein Names:DnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; HSJ-1
UniProt Gene Name:DNAJB2  
UniProt Entry Name:DNJB2_HUMAN
Anti-DNAJB2 Antibody (CAB10345)Western blot analysis of extracts of various cell lines, using DNAJB2 antibody (CAB10345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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