Anti-DNAJB1 Antibody (CAB5504)

Name: Anti-DNAJB1 Antibody (CAB5504)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5504
Availability: OutOfStock

Antibody Name:	Anti-DNAJB1 Antibody [KO Validated]
Antibody SKU:	CAB5504
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF-7, BxPC-3, BT-474, SW620, K-562, Mouse thymus, Mouse spleen, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKD YYQT LGLA RGAS DEEI KRAY RRQA LRYH PDKN KEPG AEEK FKEI AEAY DVLS DPRK REIF DRYG EEGL KGSG PSGG SGGG ANGT SFSY TFHG DPHA MFAE FFGG RNPF DTFF GQRN GEEG MDID DPFS GFPM GMGG FTNV NFGR SRSA QEPA RKKQ DPPV THDL RVSL EEIY SGCT KKMK ISHK RLNP DGKS IRNE DKIL TIEV KKGW KEGT KITF PKEG DQTS NNIP ADIV FVLK DKPH NIFK RDGS DVIY PARI SLRE ALCG CTVN VPTL DGRT IPVV FKDV IRPG MRRK VPGE GLPL PKTP EKRG DLII EFEV IFPE RIPQ TSRT VLEQ VLPI
Gene ID:	3337
Uniprot:	P25685
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	27kDa/38kDa
Observed MW:	39kDa

Synonyms:

DNAJB1, HSPF1, Hdj1, Hsp40, RSPH16B, Sis1

Background:

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
NCBI Summary:	This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:	P25685
NCBI GenInfo Identifier:	1706473
NCBI Gene ID:	3337
NCBI Accession:	P25685.4
UniProt Secondary Accession:	P25685,B4DX52,
UniProt Related Accession:	P25685
Molecular Weight:	340
NCBI Full Name:	DnaJ homolog subfamily B member 1
NCBI Synonym Full Names:	DnaJ (Hsp40) homolog, subfamily B, member 1
NCBI Official Symbol:	DNAJB1
NCBI Official Synonym Symbols:	Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
NCBI Protein Information:	dnaJ homolog subfamily B member 1; human DnaJ protein 1; dnaJ protein homolog 1; radial spoke 16 homolog B; heat shock 40 kDa protein 1
UniProt Protein Name:	DnaJ homolog subfamily B member 1
UniProt Synonym Protein Names:	DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJB1
UniProt Entry Name:	DNJB1_HUMAN

	Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (CAB5504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (CAB5504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U2OS cells using DNAJB1 antibody (CAB5504). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using DNAJB1 antibody (CAB5504) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using DNAJB1 antibody (CAB5504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
DNAJB1 Antibody (PACO00946)	Human HSP40 / DNAJB1 ELISA Kit
DNAJB1 Antibody (PACO07463)	Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit
DNAJB1 Antibody (PACO08874)	HSP40 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)