Cell Biology Antibodies 7

Anti-DNAAF1 Antibody (CAB16593)

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SKU:
CAB16593
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DNAAF1 Antibody
Antibody SKU:CAB16593
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VATE GVFV TELD GTRT EDLE TIRL ETKE TFCI DDLP DLED DDET GKSL EDQN MCFP KIEV ISSL SDDS DPEL DYTS LPVL ENLP TDTL SNIF AVSK DTSK AARV PFTD IFKK EAKR DLEI RKQD TKSP RPLI QELS DEDP SGQL LMPP TCQR DAAP LTSS GDRD SDFL AASS PVPT ESAA TPPE TCVG VAQP SQAL PTWD LTAF PAPK AS
Gene ID:123872
Uniprot:Q8NEP3
Cellular Location:Cell projection, Cytoplasm, cilium, cytoskeleton, spindle pole
Calculated MW:47kDa/53kDa/80kDa
Observed MW:80kDa
Synonyms:DNAAF1, CILD13, LRRC50, ODA7, swt
Background:The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:LRRC50: Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli. Defects in DNAAF1 are the cause of primary ciliary dyskinesia type 13 (CILD13). A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. At ultrastructural level, CILD13 is characterized by a marked reduction or absence of both dynein arms from the cilia. Belongs to the DNAAF1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: spindle pole; cytoplasm; plasma membrane; axoneme; nucleus

Molecular Function:dynein binding

Biological Process: heart looping; lung development

Disease: Ciliary Dyskinesia, Primary, 13

NCBI Summary:The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13.[provided by RefSeq, May 2010]
UniProt Code:Q8NEP3
NCBI GenInfo Identifier:157674358
NCBI Gene ID:123872
NCBI Accession:NP_848547.4
UniProt Secondary Accession:Q8NEP3,Q69YI8, Q69YJ0, Q69YW5, Q96LP3, Q96MB6, B4DJA3
UniProt Related Accession:Q8NEP3
Molecular Weight:80,026 Da
NCBI Full Name:dynein assembly factor 1, axonemal
NCBI Synonym Full Names:dynein, axonemal, assembly factor 1
NCBI Official Symbol:DNAAF1  
NCBI Official Synonym Symbols:ODA7; CILD13; LRRC50  
NCBI Protein Information:dynein assembly factor 1, axonemal; leucine rich repeat containing 50; outer row dynein assembly 7 homolog; leucine-rich repeat-containing protein 50
UniProt Protein Name:Dynein assembly factor 1, axonemal
UniProt Synonym Protein Names:Leucine-rich repeat-containing protein 50
UniProt Gene Name:DNAAF1  
UniProt Entry Name:DAAF1_HUMAN
Anti-DNAAF1 Antibody (CAB16593)Western blot analysis of extracts of rat testis, using DNAAF1 antibody (CAB16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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