Signal Transduction Antibodies 1

Anti-DMT1 Antibody (CAB10231)

(No reviews yet) Write a Review
SKU:
CAB10231
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-DMT1 Antibody
Antibody SKU:CAB10231
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DMT1 (NP_000608.1).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, BxPC-3, SH-SY5Y, Rat testis, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DMT1 (NP_000608.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVLG PEQK MSDD SVSG DHGE SASL GNIN PAYS NPSL SQSP GDSE EYFA TYFN EKIS IPEE EYSC FSFR KLWA FTGP GFLM SIAY LDPG NIES DLQS GAVA
Gene ID:4891
Uniprot:P49281
Cellular Location:Cell membrane, Early endosome, Endosome membrane, Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:61kDa/62kDa/64kDa/65kDa
Observed MW:72KDa
Synonyms:SLC11A2, AHMIO1, DCT1, DMT1, NRAMP2
Background:This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SLC11A2: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA). The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. Belongs to the NRAMP family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: apical part of cell; apical plasma membrane; brush border membrane; cytoplasm; cytoplasmic vesicle; early endosome; integral to plasma membrane; late endosome membrane; lysosomal membrane; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome; vacuole

Molecular Function:cadmium ion binding; cadmium ion transmembrane transporter activity; copper ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; ferrous iron uptake transmembrane transporter activity; iron ion transmembrane transporter activity; lead ion transmembrane transporter activity; manganese ion transmembrane transporter activity; protein binding; solute:hydrogen symporter activity; transition metal ion transmembrane transporter activity

Biological Process: cellular iron ion homeostasis; copper ion transport; ferrous iron transport; lead ion transport; manganese ion transport; response to iron ion

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 1

NCBI Summary:This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]
UniProt Code:P49281
NCBI GenInfo Identifier:8247934
NCBI Gene ID:4891
NCBI Accession:P49281.2
UniProt Secondary Accession:P49281,O43288, O60932, O94801, Q498Z5, Q8IUD7, Q96J35 B3KT08, B4DK84, F5H741,
UniProt Related Accession:P49281
Molecular Weight:61,048 Da
NCBI Full Name:Natural resistance-associated macrophage protein 2
NCBI Synonym Full Names:solute carrier family 11 member 2
NCBI Official Symbol:SLC11A2  
NCBI Official Synonym Symbols:DCT1; DMT1; AHMIO1; NRAMP2  
NCBI Protein Information:natural resistance-associated macrophage protein 2
UniProt Protein Name:Natural resistance-associated macrophage protein 2
UniProt Synonym Protein Names:Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; Solute carrier family 11 member 2
Protein Family:Natural resistance-associated macrophage protein
UniProt Gene Name:SLC11A2  
UniProt Entry Name:NRAM2_HUMAN
Anti-DMT1 Antibody (CAB10231)Western blot analysis of extracts of various cell lines, using DMT1 Rabbit pAb (CAB10231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-DMT1 Antibody (CAB10231)Immunohistochemistry of paraffin-embedded rat stomach using DMT1 Rabbit pAb (CAB10231) at dilution of 1:100 (40x lens).
Anti-DMT1 Antibody (CAB10231)Immunohistochemistry of paraffin-embedded mouse stomach using DMT1 Rabbit pAb (CAB10231) at dilution of 1:100 (40x lens).
Anti-DMT1 Antibody (CAB10231)Immunofluorescence analysis of NIH-3T3 cells using DMT1 Rabbit pAb (CAB10231) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products