Cell Cycle Antibodies 1
Anti-DLGAP5 Antibody (CAB2197)
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- SKU:
- CAB2197
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-DLGAP5 Antibody |
| Antibody SKU: | CAB2197 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 547-846 of human DLGAP5 (NP_055565.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 547-846 of human DLGAP5 (NP_055565.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KNVF RKKV VSGI ASKP KQDD AGRI AARN RLAA IKNA MRER IRQE ECAE TAVS VIPK EVDK IVFD AGFF RVES PVKL FSGL SVSS EGPS QRLG TPKS VNKA VSQS RNEM GIPQ QTTS PENA GPQN TKSE HVKK TLFL SIPE SRSS IEDA QCPG LPDL IEEN HVVN KTDL KVDC LSSE RMSL PLLA GGVA DDIN TNKK EGIS DVVE GMEL NSSI TSQD VLMS SPEK NTAS QNSI LEEG ETKI SQSE LFDN KSLT TECH LLDS PGLN CSNP FTQL ERRH QEHA RHIS FGGN LITF SPLQ PGEF |
| Gene ID: | 9787 |
| Uniprot: | Q15398 |
| Cellular Location: | Cytoplasm, Nucleus, cytoskeleton, spindle |
| Calculated MW: | 85kDa/94kDa/95kDa |
| Observed MW: | 120kDa |
| Synonyms: | DLGAP5, DLG7, HURP |
| Background: |
| UniProt Protein Function: | DLG7: a cell cycle regulator that may play a role in carcinogenesis. Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. Required for chromosome congression and alignment. Stabilizes mitotic microtubules, promotes microtubule polymerization and controls spindle formation and dynamics Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Interacts with CDC2 and localizes to the spindle poles in mitotic cells. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Colocalizes with E-cadherin (CDH1) at sites of cell-cell contact in intestinal epithelial cells. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Microtubule-binding Chromosomal Location of Human Ortholog: 14q22.3 Cellular Component: cytoplasm; microtubule organizing center; spindle pole centrosome; nucleus Molecular Function:protein binding; phosphoprotein phosphatase activity Biological Process: cell proliferation; dephosphorylation; cell-cell signaling; positive regulation of mitotic metaphase/anaphase transition; mitotic chromosome movement towards spindle pole |
| UniProt Code: | Q15398 |
| NCBI GenInfo Identifier: | 82592583 |
| NCBI Gene ID: | 9787 |
| NCBI Accession: | Q15398.2 |
| UniProt Secondary Accession: | Q15398,Q86T11, Q8NG58, A8MTM6, B4DRM8, |
| UniProt Related Accession: | Q15398 |
| Molecular Weight: | 846 |
| NCBI Full Name: | Disks large-associated protein 5 |
| NCBI Synonym Full Names: | discs, large (Drosophila) homolog-associated protein 5 |
| NCBI Official Symbol: | DLGAP5 |
| NCBI Official Synonym Symbols: | DLG7; HURP |
| NCBI Protein Information: | disks large-associated protein 5; DAP-5; discs large homolog 7; discs, large homolog 7; hepatoma up-regulated protein; disks large-associated protein DLG7 |
| UniProt Protein Name: | Disks large-associated protein 5 |
| UniProt Synonym Protein Names: | Discs large homolog 7; Disks large-associated protein DLG7; Hepatoma up-regulated protein; HURP |
| UniProt Gene Name: | DLGAP5 |
| UniProt Entry Name: | DLGP5_HUMAN |
 | Western blot analysis of extracts of K562 cells, using DLGAP5 antibody (CAB2197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using DLGAP5 antibody (CAB2197). Blue: DAPI for nuclear staining. |
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