Description
| Antibody Name: | Anti-DLG1 Antibody |
| Antibody SKU: | CAB8542 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, HeLa, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPVR KQDT QRAL HLLE EYRS KLSQ TEDR QLRS SIER VINI FQSN LFQA LIDI QEFY EVTL LDNP KCID RSKP SEPI QPVN TWEI SSLP SSTV TSET LPSS LSPS VEKY RYQD EDTP PQEH ISPQ ITNE VIGP ELVH VSEK NLSE IENV HGFV SHSH ISPI KPTE AVLP SPPT VPVI PVLP VPAE NTVI LP |
| Gene ID: | 1739 |
| Uniprot: | Q12959 |
| Cellular Location: | Basolateral cell membrane, Cell junction, Cell membrane, Endoplasmic reticulum membrane, Membrane, Peripheral membrane protein, postsynaptic cell membrane, postsynaptic density, sarcolemma, synapse |
| Calculated MW: | 87-103kDa |
| Observed MW: | 114kDa |
| Synonyms: | DLG1, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg |
| Background: | This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. |
| UniProt Protein Function: | Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. |
| NCBI Summary: | This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011] |
| UniProt Code: | Q12959 |
| NCBI GenInfo Identifier: | 223590196 |
| NCBI Gene ID: | 1739 |
| NCBI Accession: | Q12959.2 |
| UniProt Secondary Accession: | Q12959,Q12958, A5YKK7, B4DGU1, B4DGZ8, B7ZMM0, B9EIQ5 D3DXB8, D3DXB9, E7EWL7, E9PG21, |
| UniProt Related Accession: | Q12959 |
| Molecular Weight: | 100kDa |
| NCBI Full Name: | Disks large homolog 1 |
| NCBI Synonym Full Names: | discs large MAGUK scaffold protein 1 |
| NCBI Official Symbol: | DLG1 |
| NCBI Official Synonym Symbols: | hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1 |
| NCBI Protein Information: | disks large homolog 1 |
| UniProt Protein Name: | Disks large homolog 1 |
| UniProt Synonym Protein Names: | Synapse-associated protein 97; SAP-97; SAP97; hDlg |
| Protein Family: | Disks large |
| UniProt Gene Name: | DLG1 |
 | Western blot analysis of extracts of various cell lines, using DLG1 antibody (CAB8542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using DLG1 Rabbit pAb (CAB8542) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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