Metabolism Antibodies 2

Anti-DKK4 Antibody (CAB7797)

(No reviews yet) Write a Review
SKU:
CAB7797
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-DKK4 Antibody
Antibody SKU:CAB7797
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-224 of human DKK4 (NP_055235.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:SW480, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-224 of human DKK4 (NP_055235.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LVLD FNNI RSSA DLHG ARKG SQCL SDTD CNTR KFCL QPRD EKPF CATC RGLR RRCQ RDAM CCPG TLCV NDVC TTME DATP ILER QLDE QDGT HAEG TTGH PVQE NQPK RKPS IKKS QGRK GQEG ESCL RTFD CGPG LCCA RHFW TKIC KPVL LEGQ VCSR RGHK DTAQ APEI FQRC DCGP GLLC RSQL TSNR QHAR LRVC QKIE KL
Gene ID:27121
Uniprot:Q9UBT3
Cellular Location:Secreted
Calculated MW:24kDa
Observed MW:25-30kDa
Synonyms:DKK4, DKK-4
Background:This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2.
UniProt Protein Function:DKK4: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.
UniProt Protein Details:

Protein type:Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p11.2-p11.1

Cellular Component: extracellular region

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development

NCBI Summary:This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UBT3
NCBI GenInfo Identifier:13124092
NCBI Gene ID:27121
NCBI Accession:Q9UBT3.1
UniProt Secondary Accession:Q9UBT3,Q3KNX0, Q9Y4C3,
UniProt Related Accession:Q9UBT3
Molecular Weight:24,876 Da
NCBI Full Name:Dickkopf-related protein 4
NCBI Synonym Full Names:dickkopf WNT signaling pathway inhibitor 4
NCBI Official Symbol:DKK4  
NCBI Official Synonym Symbols:DKK-4  
NCBI Protein Information:dickkopf-related protein 4
UniProt Protein Name:Dickkopf-related protein 4
Protein Family:Dickkopf-related protein
UniProt Gene Name:DKK4  
UniProt Entry Name:DKK4_HUMAN
Anti-DKK4 Antibody (CAB7797)Western blot analysis of extracts of various cell lines, using DKK4 antibody (CAB7797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products