Metabolism Antibodies 2
Anti-DKK3 Antibody (CAB2527)
- SKU:
- CAB2527
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DKK3 Antibody |
| Antibody SKU: | CAB2527 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-350 of human DKK3 (NP_037385.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-350 of human DKK3 (NP_037385.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APAP TATS APVK PGPA LSYP QEEA TLNE MFRE VEEL MEDT QHKL RSAV EEME AEEA AAKA SSEV NLAN LPPS YHNE TNTD TKVG NNTI HVHR EIHK ITNN QTGQ MVFS ETVI TSVG DEEG RRSH ECII DEDC GPSM YCQF ASFQ YTCQ PCRG QRML CTRD SECC GDQL CVWG HCTK MATR GSNG TICD NQRD CQPG LCCA FQRG LLFP VCTP LPVE GELC HDPA SRLL DLIT WELE PDGA LDRC PCAS GLLC QPHS HSLV YVCK PTFV GSRD QDGE ILLP REVP DEYE VGSF MEEV RQEL EDLE RSLT EEMA LREP AAAA AALL GGEE I |
| Gene ID: | 27122 |
| Uniprot: | Q9UBP4 |
| Cellular Location: | Secreted |
| Calculated MW: | 38kDa |
| Observed MW: | 38kDa |
| Synonyms: | DKK3, REIC, RIG |
| Background: | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. |
| UniProt Protein Function: | DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: extracellular space Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBP4 |
| NCBI GenInfo Identifier: | 311033372 |
| NCBI Gene ID: | 27122 |
| NCBI Accession: | Q9UBP4.2 |
| UniProt Secondary Accession: | Q9UBP4,Q9ULB7, A8K1I2, D3DQW1, |
| UniProt Related Accession: | Q9UBP4 |
| Molecular Weight: | 38,390 Da |
| NCBI Full Name: | Dickkopf-related protein 3 |
| NCBI Synonym Full Names: | dickkopf WNT signaling pathway inhibitor 3 |
| NCBI Official Symbol: | DKK3 |
| NCBI Official Synonym Symbols: | RIG; REIC |
| NCBI Protein Information: | dickkopf-related protein 3; dkk-3; hDkk-3; dickkopf-3; RIG-like 5-6; RIG-like 7-1; dickkopf 3 homolog; dickkopf homolog 3; regulated in glioma |
| UniProt Protein Name: | Dickkopf-related protein 3 |
| Protein Family: | Dickkopf-related protein |
| UniProt Gene Name: | DKK3 |
| UniProt Entry Name: | DKK3_HUMAN |
 | Western blot analysis of extracts of mouse brain, using DKK3 antibody (CAB2527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using DKK3 antibody (CAB2527) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using DKK3 antibody (CAB2527) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using DKK3 Polyclonal Antibody (CAB2527) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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