Name: Anti-DISP1 Antibody (CAB14946)
Brand: Cell Biology Antibodies 5
SKU: CAB14946
Availability: OutOfStock

Antibody Name:	Anti-DISP1 Antibody
Antibody SKU:	CAB14946
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1325-1524 of human DISP1 (NP_116279.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HT-29, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1325-1524 of human DISP1 (NP_116279.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FVHP ITHI HHCP CLQG RVKP AGMQ NSLP RNFF LHPV QHIQ AQEK IGKT NVHS LQRS IEEH LPKM AEPS SFVC RSTG SLLK TCCD PENK QREL CKNR DVSN LESS GGTE NKAG GKVE LSLS QTDA SVNS EHFN QNEP KVLF NHLM GEAG CRSC PNNS QSCG RIVR VKCN SVDC QMPN MEAN VPAV LTHS ELSG ESLL IKTL
Gene ID:	84976
Uniprot:	Q96F81
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	170kDa
Observed MW:	180kDa

Synonyms:

DISP1, DISPA

Background:

The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched and, based on sequence identity to its mouse counterpart, the encoded protein may play an essential role in Hh patterning activities in the early embryo.

UniProt Protein Function:	Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal. Synergizes with SCUBE2 to cause a increase in SHH secretion (PubMed:22902404).
NCBI Summary:	The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched and, based on sequence identity to its mouse counterpart, the encoded protein may play an essential role in Hh patterning activities in the early embryo. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96F81
NCBI GenInfo Identifier:	25952134
NCBI Gene ID:	84976
NCBI Accession:	NP_116279.2
UniProt Secondary Accession:	Q96F81,Q8N7C2, Q96I92, Q9H698, Q9H8H9, Q9UFA2,
UniProt Related Accession:	Q96F81
Molecular Weight:	167
NCBI Full Name:	protein dispatched homolog 1 isoform 1
NCBI Synonym Full Names:	dispatched RND transporter family member 1
NCBI Official Symbol:	DISP1
NCBI Official Synonym Symbols:	DISPA
NCBI Protein Information:	protein dispatched homolog 1
UniProt Protein Name:	Protein dispatched homolog 1
Protein Family:	Protein dispatched
UniProt Gene Name:	DISP1

Western blot analysis of extracts of various cell lines, using DISP1 antibody (CAB14946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

DISP1 Antibody (PACO08845)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)