Name: Anti-DIS3 Antibody (CAB8027)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB8027
Availability: OutOfStock

Antibody Name:	DIS3 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8027
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DIS3 (NP_055768.3).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, DU145, SGC-7901

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DIS3 (NP_055768.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLKS KTFL KKTR AGGV MKIV REHY LRDD IGCG APGC AACG GAHE GPAL EPQP QDPA SSVC PQPH YLLP DTNV LLHQ IDVL EDPA IRNV IVLQ TVLQ EVRN RSAP VYKR IRDV TNNQ EKHF YTFT NEHH RETY VEQE QGEN ANDR NDRA IRVA AKWY NEHL KKMS ADNQ LQVI FITN DRRN KEKA IEEG IPAF TCEE YVKS LTAN PELI DRLA CLSE EGNE IESG KIIF SEHL PLSK LQQG IKSG TYLQ GTFR ASRE NYLE ATVW IHGD NEEN KEII LQGL
Gene ID:	22894
Uniprot:	Q9Y2L1
Cellular Location:	Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:	105kDa/109kDa
Observed MW:	109kDa

Synonyms:	DIS3, 2810028N01Rik, EXOSC11, KIAA1008, RRP44, dis3p
Background:

UniProt Protein Function:	DIS3: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. Belongs to the RNR ribonuclease family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.26.-; EC 3.1.13.-; Ribonuclease; Nucleolus; G protein regulator, misc.; EC 3.1.13.-; RNA processing Chromosomal Location of Human Ortholog: 13q22.1 Cellular Component: nucleoplasm; membrane; nucleolus; exosome (RNase complex); cytosol; nuclear exosome (RNase complex) Molecular Function:3'-5'-exoribonuclease activity; protein binding; RNA binding; guanyl-nucleotide exchange factor activity; endonuclease activity Biological Process: rRNA catabolic process; gene expression; rRNA processing; mRNA catabolic process, deadenylation-dependent decay; positive regulation of GTPase activity
UniProt Code:	Q9Y2L1
NCBI GenInfo Identifier:	190014625
NCBI Gene ID:	22894
NCBI Accession:	NP_001121698.1
UniProt Secondary Accession:	Q9Y2L1,Q5W0P7, Q5W0P8, Q658Z7, Q7Z481, Q8WWI2, Q9UG36 A6NI21, B2RBL2,
UniProt Related Accession:	Q9Y2L1
Molecular Weight:	109,003 Da
NCBI Full Name:	exosome complex exonuclease RRP44 isoform b
NCBI Synonym Full Names:	DIS3 exosome endoribonuclease and 3'-5' exoribonuclease
NCBI Official Symbol:	DIS3
NCBI Official Synonym Symbols:	RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik
NCBI Protein Information:	exosome complex exonuclease RRP44; exosome component 11; DIS3 mitotic control homolog; ribosomal RNA-processing protein 44; mitotic control protein dis3 homolog
UniProt Protein Name:	Exosome complex exonuclease RRP44
UniProt Synonym Protein Names:	Protein DIS3 homolog; Ribosomal RNA-processing protein 44
Protein Family:	Exosome complex exonuclease
UniProt Gene Name:	DIS3
UniProt Entry Name:	RRP44_HUMAN

Western blot analysis of extracts of various cell lines, using DIS3 antibody (CAB8027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

DIS3 Antibody (PACO08844)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)