Cell Biology Antibodies 11

Anti-DIRAS3 Antibody (CAB7950)

(No reviews yet) Write a Review
SKU:
CAB7950
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-DIRAS3 Antibody
Antibody SKU:CAB7950
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human DIRAS3 (NP_004666.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3, Mouse liver, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human DIRAS3 (NP_004666.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGNA SFGS KEQK LLKR LRLL PALL ILRA FKPH RKIR DYRV VVVG TAGV GKST LLHK WASG NFRH EYLP TIEN TYCQ LLGC SHGV LSLH ITDS KSGD GNRA LQRH VIAR GHAF VLVY SVTK KETL EELK AFYE LICK IKGN NLHK FPIV LVGN KSDD THRE VALN DGAT CAME WNCA FMEI SAKT DVNV QELF HMLL NYKK KPTT GLQE PEKK SQMP NTTE KLLD KCII M
Gene ID:9077
Uniprot:O95661
Cellular Location:Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:25kDa
Observed MW:30kDa
Synonyms:DIRAS3, ARHI, NOEY2
Background:This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex.
UniProt Protein Function:DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]
UniProt Protein Details:

Protein type:G protein, monomeric; G protein; G protein, monomeric, di-Ras

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: plasma membrane

Molecular Function:GTPase activity; protein binding; GTP binding

Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity

UniProt Code:O95661
NCBI GenInfo Identifier:49456739
NCBI Gene ID:
NCBI Accession:CAG46690.1
UniProt Secondary Accession:O95661,B3KMP3,
Molecular Weight:25 kDa
NCBI Full Name:ARHI, partial
UniProt Protein Name:GTP-binding protein Di-Ras3
UniProt Synonym Protein Names:Distinct subgroup of the Ras family member 3; Rho-related GTP-binding protein RhoI
UniProt Gene Name:DIRAS3  
UniProt Entry Name:DIRA3_HUMAN
Anti-DIRAS3 Antibody (CAB7950)Western blot analysis of extracts of various cell lines, using DIRAS3 antibody (CAB7950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products