Metabolism Antibodies 2
Anti-DIO3 Antibody (CAB6900)
- SKU:
- CAB6900
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DIO3 Antibody |
| Antibody SKU: | CAB6900 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human DIO3 (NP_001353.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SW620, HepG2, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human DIO3 (NP_001353.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HFLG RRRR GQPE PEVE LNSE GEEV PPDD PPIC VSDD NRLC TLAS LKAV WHGQ KLDF FKQA HEGG PAPN SEVV LPDG FQSQ HILD YAQG NRPL VLNF GSCT U |
| Gene ID: | 1735 |
| Uniprot: | P55073 |
| Cellular Location: | Cell membrane, Endosome membrane, Single-pass type II membrane protein |
| Calculated MW: | 33kDa |
| Observed MW: | 34kDa |
| Synonyms: | DIO3, 5DIII, D3, DIOIII, TXDI3 |
| Background: | The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3, 3', 5-triiodothyronine (T3) to inactive metabolites, 3, 3', 5'-triiodothyronine (RT3) and 3, 3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. |
| UniProt Protein Function: | DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family. |
| UniProt Protein Details: | Protein type:EC 1.97.1.11; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 14q32 Cellular Component: integral to membrane; plasma membrane; endosome membrane Molecular Function:thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity Biological Process: thyroid hormone catabolic process; thyroid hormone generation; hormone biosynthetic process; positive regulation of multicellular organism growth |
| NCBI Summary: | The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55073 |
| NCBI GenInfo Identifier: | 635377461 |
| NCBI Gene ID: | 1735 |
| NCBI Accession: | P55073.4 |
| UniProt Secondary Accession: | P55073,Q8WVN5, G3XAM0, |
| UniProt Related Accession: | P55073 |
| Molecular Weight: | 278 |
| NCBI Full Name: | Type III iodothyronine deiodinase |
| NCBI Synonym Full Names: | deiodinase, iodothyronine, type III |
| NCBI Official Symbol: | DIO3 |
| NCBI Official Synonym Symbols: | D3; 5DIII; TXDI3; DIOIII |
| NCBI Protein Information: | type III iodothyronine deiodinase; type 3 DI; type-III 5' deiodinase; type-III 5'-deiodinase; type 3 iodothyronine selenodeiodinase; iodothyronine deiodinase, placental type; thyroxine deiodinase type III (selenoprotein) |
| UniProt Protein Name: | Type III iodothyronine deiodinase |
| UniProt Synonym Protein Names: | 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase |
| Protein Family: | Thyroxine 5-deiodinase |
| UniProt Gene Name: | DIO3 |
| UniProt Entry Name: | IOD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DIO3 antibody (CAB6900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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