Epigenetics & Nuclear Signaling Antibodies 4

Anti-DIMT1 Antibody (CAB7589)

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SKU:
CAB7589
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DIMT1 Antibody
Antibody SKU:CAB7589
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human DIMT1 (NP_055288.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:H460, U-87MG, HeLa, MCF7, K-562, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human DIMT1 (NP_055288.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPKV KSGA IGRR RGRQ EQRR ELKS AGGL MFNT GIGQ HILK NPLI INSI IDKA ALRP TDVV LEVG PGTG NMTV KLLE KAKK VVAC ELDP RLVA ELHK RVQG TPVA SKLQ VLVG DVLK TDLP FFDT CV
Gene ID:27292
Uniprot:Q9UNQ2
Cellular Location:Nucleus, nucleolus
Calculated MW:35kDa
Observed MW:35kDa
Synonyms:DIMT1, DIM1, DIMT1L, HSA9761, HUSSY5
Background:
UniProt Protein Function:DIMT1: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.
UniProt Protein Details:

Protein type:EC 2.1.1.183; Nucleolus; RNA-binding; Methyltransferase

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function:rRNA (adenine-N6,N6-)-dimethyltransferase activity

Biological Process: rRNA methylation

NCBI Summary:The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017]
UniProt Code:Q9UNQ2
NCBI GenInfo Identifier:27151492
NCBI Gene ID:27292
NCBI Accession:Q9UNQ2.1
UniProt Related Accession:Q9UNQ2
Molecular Weight:35 kDa
NCBI Full Name:Probable dimethyladenosine transferase
NCBI Synonym Full Names:DIMT1 rRNA methyltransferase and ribosome maturation factor
NCBI Official Symbol:DIMT1  
NCBI Official Synonym Symbols:DIM1; DIMT1L; HUSSY5; HSA9761  
NCBI Protein Information:probable dimethyladenosine transferase
UniProt Protein Name:Probable dimethyladenosine transferase
UniProt Synonym Protein Names:DIM1 dimethyladenosine transferase 1 homolog; DIM1 dimethyladenosine transferase 1-like; Probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; Probable 18S rRNA dimethylase; Probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase
Protein Family:Probable dimethyladenosine transferase
UniProt Gene Name:DIMT1  
UniProt Entry Name:DIM1_HUMAN
Anti-DIMT1 Antibody (CAB7589)Western blot analysis of extracts of various cell lines, using DIMT1 antibody (CAB7589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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