Antibody Name:	Anti-DiMethyl-DNMT3A-K44 Antibody
Antibody SKU:	CAB16012
Antibody Size:	20uL, 50uL, 100uL
Application:	IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).

Application:	IF
Recommended Dilution:	IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARKV G
Gene ID:	13435
Uniprot:	O88508
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	77kDa/101kDa
Observed MW:

Synonyms:

Dnmt3a, MmuIIIA, DNMT3A2, M.HsaIIIA, TBRS

Background:

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

UniProt Protein Function:	DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
UniProt Protein Details:	Protein type:Methyltransferase, DNA; EC 2.1.1.37; Methyltransferase; Amino Acid Metabolism - cysteine and methionine Cellular Component: nucleoplasm; XY body; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region Molecular Function:transferase activity; DNA (cytosine-5-)-methyltransferase activity; methyltransferase activity; identical protein binding; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding Biological Process: methylation; genetic imprinting; maintenance of DNA methylation; transcription, DNA-dependent; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; regulation of transcription, DNA-dependent; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; DNA methylation during embryonic development; DNA methylation on cytosine
NCBI Summary:	This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
UniProt Code:	O88508
NCBI GenInfo Identifier:	418203928
NCBI Gene ID:	13435
NCBI Accession:	NP_001258682.1
UniProt Related Accession:	O88508
Molecular Weight:	77kDa; 101kDa
NCBI Full Name:	DNA (cytosine-5)-methyltransferase 3A isoform 1
NCBI Synonym Full Names:	DNA methyltransferase 3A
NCBI Official Symbol:	Dnmt3a
NCBI Official Synonym Symbols:	MmuIIIA
NCBI Protein Information:	DNA (cytosine-5)-methyltransferase 3A
UniProt Protein Name:	DNA (cytosine-5)-methyltransferase 3A
UniProt Synonym Protein Names:	DNA methyltransferase MmuIIIA; DNA MTase MmuIIIA; M.MmuIIIA
Protein Family:	DNA
UniProt Gene Name:	Dnmt3a
UniProt Entry Name:	DNM3A_MOUSE

	Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody (CAB16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody (CAB16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody (CAB16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (CAB16012) at dilution of 1:100. Blue: DAPI for nuclear staining.