Name: Anti-DHX58 Antibody (CAB8257)
Brand: Immunology Antibodies 3
SKU: CAB8257
Availability: OutOfStock

Antibody Name:	DHX58 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8257
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QQQG LQTV DIRA QLLI GAGN SSQS THMT QRDQ QEVI QKFQ DGTL NLLV ATSV AEEG LDIP HCNV VVRY GLLT NEIS MVQA RGRA RADQ SVYA FVAT EGSR ELKR ELIN EALE TLME QAVA AVQK MDQA EYQA KIRD LQQA ALTK RAAQ AAQR ENQR QQFP VEHV QLLC INCM VAVG HGSD LRKV EGTH HVNV NPNF SNYY NVSR DPVV INKV FKDW KPGG VISC RNCG EVWG LQMI YKSV KLPV LKVR SMLL ETPQ GRIQ AKKW SRVP FSVP DFDF LQHC AENL SDLS LD
Gene ID:	79132
Uniprot:	Q96C10
Cellular Location:	Cytoplasm
Calculated MW:	76kDa
Observed MW:	77kDa

Synonyms:	DHX58, D11LGP2, D11lgp2e, LGP2, RLR-3
Background:

UniProt Protein Function:	DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily.
UniProt Protein Details:	Protein type:Helicase; Inhibitor; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm Molecular Function:ATP binding; DNA binding; double-stranded RNA binding; helicase activity; protein binding; single-stranded RNA binding; zinc ion binding Biological Process: defense response to virus; innate immune response; metabolic process; negative regulation of innate immune response; negative regulation of interferon type I production; positive regulation of interferon type I production; regulation of innate immune response; response to virus; viral reproduction
UniProt Code:	Q96C10
NCBI GenInfo Identifier:	50401123
NCBI Gene ID:	79132
NCBI Accession:	Q96C10.1
UniProt Secondary Accession:	Q96C10,Q9HAM6,
UniProt Related Accession:	Q96C10
Molecular Weight:	76,613 Da
NCBI Full Name:	Probable ATP-dependent RNA helicase DHX58
NCBI Synonym Full Names:	DEXH-box helicase 58
NCBI Official Symbol:	DHX58
NCBI Official Synonym Symbols:	LGP2; RLR-3; D11LGP2; D11lgp2e
NCBI Protein Information:	probable ATP-dependent RNA helicase DHX58
UniProt Protein Name:	Probable ATP-dependent RNA helicase DHX58
UniProt Synonym Protein Names:	Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR
UniProt Gene Name:	DHX58
UniProt Entry Name:	DHX58_HUMAN

	Western blot analysis of extracts of various cell lines, using DHX58 antibody (CAB8257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using DHX58 antibody (CAB8257) at dilution of 1:100. Blue: DAPI for nuclear staining.