Cell Biology Antibodies 12

Anti-DHX30 Antibody (CAB8575)

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SKU:
CAB8575
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DHX30 Antibody
Antibody SKU:CAB8575
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 906-1155 of human DHX30 (NP_055781.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, LO2, MCF7, Mouse eye, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 906-1155 of human DHX30 (NP_055781.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GWEE VLRW QDRS SREN YLEE NLLY APSL RFIH GLIK QFSE NIYE AFLV GKPS DCTL ASAQ CNEY SEEE ELVK GVLM AGLY PNLI QVRQ GKVT RQGK FKPN SVTY RTKS GNIL LHKS TINR EATR LRSR WLTY FMAV KSNG SVFV RDSS QVHP LAVL LLTD GDVH IRDD GRRA TISL SDSD LLRL EGDS RTVR LLKE LRRA LGRM VERS LRSE LAAL PPSV QEEH GQLL ALLA ELLR GPCG SFDV RKTA DD
Gene ID:22907
Uniprot:Q7L2E3
Cellular Location:Cytoplasm, Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid
Calculated MW:129kDa/133kDa/136kDa
Observed MW:129kDa
Synonyms:DHX30, DDX30, RETCOR
Background:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:DDX30: Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Helicase; RNA-binding; Nuclear receptor co-regulator; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytoplasm; mitochondrion; nucleus

Molecular Function:ATP-dependent RNA helicase activity; chromatin binding; double-stranded RNA binding; protein binding

Biological Process: RNA processing

NCBI Summary:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
UniProt Code:Q7L2E3
NCBI GenInfo Identifier:74758997
NCBI Gene ID:22907
NCBI Accession:Q7L2E3.1
UniProt Secondary Accession:Q7L2E3,O94965, Q7Z753, Q96CH4, Q9NUQ0, A8K5F1,
UniProt Related Accession:Q7L2E3
Molecular Weight:129,438 Da
NCBI Full Name:Putative ATP-dependent RNA helicase DHX30
NCBI Synonym Full Names:DEAH-box helicase 30
NCBI Official Symbol:DHX30  
NCBI Official Synonym Symbols:DDX30; RETCOR  
NCBI Protein Information:putative ATP-dependent RNA helicase DHX30
UniProt Protein Name:Putative ATP-dependent RNA helicase DHX30
UniProt Synonym Protein Names:DEAH box protein 30
Protein Family:Putative ATP-dependent RNA helicase
UniProt Gene Name:DHX30  
UniProt Entry Name:DHX30_HUMAN
Anti-DHX30 Polyclonal Antibody (CAB8575)Western blot analysis of extracts of various cell lines, using DHX30 antibody (CAB8575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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