Metabolism Antibodies 2

Anti-DHRS9 Antibody (CAB6324)

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SKU:
CAB6324
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-DHRS9 Antibody
Antibody SKU:CAB6324
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:THP-1, Mouse trachea, HT-29, Mouse pancreas, Rat trachea
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RKGK LKIE DITD KYIF ITGC DSGF GNLA ARTF DKKG FHVI AACL TESG STAL KAET SERL RTVL LDVT DPEN VKRT AQWV KNQV GEKG LWGL INNA GVPG VLAP TDWL TLED YREP IEVN LFGL ISVT LNML PLVK KAQG RVIN VSSV GGRL AIVG GGYT PSKY AVEG FNDS LRRD MKAF GVHV SCIE PGLF KTNL ADPV KVIE KKLA IWEQ LSPD IKQQ YGEG YIEK SLDK LKGN KSYV NMDL SPVV ECMD HALT SLFP KTHY AAGK DAKI FWIP LSHM PAAL QDFL LLKQ KAEL ANPK AV
Gene ID:10170
Uniprot:Q9BPW9
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane
Calculated MW:18kDa/30kDa/35kDa/41kDa
Observed MW:35kDa
Synonyms:DHRS9, 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4
Background:This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:DHRS9: 3-alpha-hydroxysteroid dehydrogenase that converts 3- alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Secreted; EC 1.1.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function:alcohol dehydrogenase activity; racemase and epimerase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; retinol dehydrogenase activity

Biological Process: epithelial cell differentiation; retinol metabolic process; androgen metabolic process; 9-cis-retinoic acid biosynthetic process; progesterone metabolic process

NCBI Summary:This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:Q9BPW9
NCBI GenInfo Identifier:74752227
NCBI Gene ID:10170
NCBI Accession:Q9BPW9.1
UniProt Secondary Accession:Q9BPW9,Q5RKX1, Q9NRA9, Q9NRB0, B7Z416, D3DPC1,
UniProt Related Accession:Q9BPW9
Molecular Weight:319
NCBI Full Name:Dehydrogenase/reductase SDR family member 9
NCBI Synonym Full Names:dehydrogenase/reductase (SDR family) member 9
NCBI Official Symbol:DHRS9  
NCBI Official Synonym Symbols:RDHL; RDH15; RDH-E2; RDHTBE; SDR9C4; RDH-TBE; RETSDR8; 3ALPHA-HSD; 3-alpha-HSD  
NCBI Protein Information:dehydrogenase/reductase SDR family member 9; retinol dehydrogenase homolog; 3-alpha hydroxysteroid dehydrogenase; short-chain dehydrogenase/reductase retSDR8; NADP-dependent retinol dehydrogenase/reductase; short chain dehydrogenase/reductase family 9C, member 4; tracheobronchial epithelial cell-specific retinol dehydrogenase
UniProt Protein Name:Dehydrogenase/reductase SDR family member 9
UniProt Synonym Protein Names:3-alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; RDHL; Short-chain dehydrogenase/reductase retSDR8; Tracheobronchial epithelial cell-specific retinol dehydrogenase; RDH-TBE
Protein Family:Dehydrogenase/reductase SDR family
UniProt Gene Name:DHRS9  
UniProt Entry Name:DHRS9_HUMAN
Anti-DHRS9 Antibody (CAB6324)Western blot analysis of extracts of various cell lines, using DHRS9 antibody (CAB6324) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 390s.
Anti-DHRS9 Antibody (CAB6324)Western blot analysis of extracts of various cell lines, using DHRS9 antibody (CAB6324) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
Anti-DHRS9 Antibody (CAB6324)Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (CAB6324) at dilution of 1:100 (40x lens).
Anti-DHRS9 Antibody (CAB6324)Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (CAB6324) at dilution of 1:100 (40x lens).
Anti-DHRS9 Antibody (CAB6324)Immunofluorescence analysis of U2OS cells using DHRS9 antibody (CAB6324). Blue: DAPI for nuclear staining.
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