Cell Biology Antibodies 1

Anti-DHRS4 Antibody (CAB10285)

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SKU:
CAB10285
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DHRS4 Antibody
Antibody SKU:CAB10285
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human DHRS4 (NP_066284.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, LO2, A-549, MCF7, SW620, Mouse liver, Mouse lung, Mouse small intestine, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human DHRS4 (NP_066284.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MHKA GLLG LCAR AWNS VRMA SSGM TRRD PLAN KVAL VTAS TDGI GFAI ARRL AQDG AHVV VSSR KQQN VDQA VATL QGEG LSVT GTVC HVGK AEDR ERLV ATAV KLHG GIDI LVSN AAVN PFFG SIMD VTEE VWDK TLDI NVKA PALM TKAV VPEM EKRG GGSV VIVS SIAA FSPS PGFS PYNV SKTA LLGL TKTL AIEL APRN IRVN CLAP GLIK TSFS RMLW MDKE KEES MKET LRIR RLGE PEDC AGIV SFLC SEDA SYIT GETV VVGG GTPS RL
Gene ID:10901
Uniprot:Q9BTZ2
Cellular Location:Nucleus, Peroxisome
Calculated MW:8kDa/16kDa/20kDa/21kDa/23kDa/25kDa/29kDa
Observed MW:30kDa
Synonyms:DHRS4, CR, NRDR, PHCR, PSCD, SCAD-SRL, SDR-SRL, SDR25C1, SDR25C2
Background:
UniProt Protein Function:DHRS4: Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co- factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.1.1.184; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; nuclear membrane; mitochondrion; intracellular membrane-bound organelle; peroxisome; nucleus

Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; alcohol dehydrogenase [NAD(P)+] activity; 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity; receptor binding

Biological Process: steroid metabolic process; ketone metabolic process; alcohol metabolic process; protein tetramerization

UniProt Code:Q9BTZ2
NCBI GenInfo Identifier:308153604
NCBI Gene ID:10901
NCBI Accession:Q9BTZ2.3
UniProt Secondary Accession:Q9BTZ2,O95162, Q20CR0, Q2LC19, Q2LE81, Q58IU4, Q6E0Y1 Q6UWU3, B2RB10, B7WNS9, D3YTB8, E2QRL8,
UniProt Related Accession:Q9BTZ2
Molecular Weight:23,917 Da
NCBI Full Name:Dehydrogenase/reductase SDR family member 4
NCBI Synonym Full Names:dehydrogenase/reductase (SDR family) member 4
NCBI Official Symbol:DHRS4  
NCBI Official Synonym Symbols:CR; NRDR; PHCR; PSCD; SDR-SRL; SDR25C1; SDR25C2; SCAD-SRL  
NCBI Protein Information:dehydrogenase/reductase SDR family member 4; peroxisomal short-chain alcohol dehydrogenase; NADPH-dependent retinol dehydrogenase/reductase; short-chain dehydrogenase/reductase family member 4; dehydrogenase/reductase (SDR family) member 4 like 2A; short chain dehydrogenase/reductase family 25C, member 1; short chain dehydrogenase/reductase family 25C, member 2; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
UniProt Protein Name:Dehydrogenase/reductase SDR family member 4
UniProt Synonym Protein Names:NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short-chain dehydrogenase/reductase family member 4
UniProt Gene Name:DHRS4  
UniProt Entry Name:DHRS4_HUMAN
Anti-DHRS4 Antibody (CAB10285)Western blot analysis of extracts of various cell lines, using DHRS4 antibody (CAB10285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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