Cell Biology Antibodies 4
Anti-DHRS3 Antibody (CAB14172)
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- SKU:
- CAB14172
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DHRS3 Antibody |
| Antibody SKU: | CAB14172 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-150 of human DHRS3 (NP_004744.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse lung, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-150 of human DHRS3 (NP_004744.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PAKL RDLS RENV LITG GGRG IGRQ LARE FAER GARK IVLW GRTE KCLK ETTE EIRQ MGTE CHYF ICDV GNRE EVYQ TAKA VREK VGDI TILV NNAA VVHG KSLM DSDD DALL KSQH INTL G |
| Gene ID: | 9249 |
| Uniprot: | O75911 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 18kDa/33kDa |
| Observed MW: | 33kDa |
| Synonyms: | DHRS3, DD83.1, RDH17, Rsdr1, SDR1, SDR16C1, retSDR1 |
| Background: |
| UniProt Protein Function: | DHRS3: Catalyzes the reduction of all-trans-retinal to all- trans-retinol in the presence of NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; Membrane protein, multi-pass; EC 1.1.1.300 Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: endoplasmic reticulum membrane Molecular Function:electron carrier activity; nucleotide binding Biological Process: retinoid metabolic process; retinol metabolic process; visual perception |
| NCBI Summary: | Short-chain dehydrogenases/reductases (SDRs), such as DHRS3, catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids (Haeseleer and Palczewski, 2000 [PubMed 10800688]).[supplied by OMIM, Jun 2009] |
| UniProt Code: | O75911 |
| NCBI GenInfo Identifier: | 62298137 |
| NCBI Gene ID: | 9249 |
| NCBI Accession: | O75911.2 |
| UniProt Secondary Accession: | O75911,Q5VUY3, Q6UY38, Q9BUC8, B2R7F3, |
| UniProt Related Accession: | O75911 |
| Molecular Weight: | 18,675 Da |
| NCBI Full Name: | Short-chain dehydrogenase/reductase 3 |
| NCBI Synonym Full Names: | dehydrogenase/reductase 3 |
| NCBI Official Symbol: | DHRS3 |
| NCBI Official Synonym Symbols: | SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1 |
| NCBI Protein Information: | short-chain dehydrogenase/reductase 3 |
| UniProt Protein Name: | Short-chain dehydrogenase/reductase 3 |
| UniProt Synonym Protein Names: | DD83.1; Retinal short-chain dehydrogenase/reductase 1; retSDR1; Retinol dehydrogenase 17; Short chain dehydrogenase/reductase family 16C member 1 |
| Protein Family: | Short-chain dehydrogenase/reductase |
| UniProt Gene Name: | DHRS3 |
| UniProt Entry Name: | DHRS3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHRS3 antibody (CAB14172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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