Name: Anti-DHPS Antibody (CAB6367)
Brand: Metabolism Antibodies 2
SKU: CAB6367
Availability: OutOfStock

Antibody Name:	Anti-DHPS Antibody
Antibody SKU:	CAB6367
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DHPS (NP_001921.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, BT-474, 293T, Jurkat, SGC-7901, Mouse testis, Mouse brain, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DHPS (NP_001921.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEGS LERE APAG ALAA VLKH SSTL PPES TQVR GYDF NRGV NYRA LLEA FGTT GFQA TNFG RAVQ QVNA MIEK KLEP LSQD EDQH ADLT QSRR PLTS CTIF LGYT SNLI SSGI RETI RYLV QHNM VDVL VTTA GGVE EDLI KCLA PTYL GEFS LRGK ELRE NGIN RIGN LLVP NENY CKFE DWLM PILD QMVM EQNT EGVK WTPS KMIA RLGK EINN PESV YYWA QKNH IPVF SPAL TDGS LGDM IFFH SYKN PGLV LDIV EDLR LINT QAIF AKCT GMII LGGG VVKH HIAN ANLM RNGA DYAV YINT AQEF DGSD SGAR PDEA VSWG KIRV DAQP VKVY ADAS LVFP LLVA ETFA QKMD AFMH EKNE D
Gene ID:	1725
Uniprot:	P49366
Cellular Location:
Calculated MW:	35kDa/36kDa/40kDa
Observed MW:	41kDa

Synonyms:

DHPS, DHS, DS, MIG13

Background:

This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	DHPS: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Belongs to the deoxyhypusine synthase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; EC 2.5.1.46; Transferase Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: cytosol Molecular Function:deoxyhypusine synthase activity; protein binding Biological Process: hypusine biosynthetic process from peptidyl-lysine; positive regulation of cell proliferation; translation
NCBI Summary:	This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]
UniProt Code:	P49366
NCBI GenInfo Identifier:	1352267
NCBI Gene ID:	1725
NCBI Accession:	P49366.1
UniProt Secondary Accession:	P49366,Q13184, Q13276, Q9UDG0, A8K688, M0R1I5,
UniProt Related Accession:	P49366
Molecular Weight:	36,583 Da
NCBI Full Name:	Deoxyhypusine synthase
NCBI Synonym Full Names:	deoxyhypusine synthase
NCBI Official Symbol:	DHPS
NCBI Official Synonym Symbols:	DS; DHS; MIG13
NCBI Protein Information:	deoxyhypusine synthase
UniProt Protein Name:	Deoxyhypusine synthase
UniProt Gene Name:	DHPS
UniProt Entry Name:	DHYS_HUMAN

Western blot analysis of extracts of various cell lines, using DHPS antibody (CAB6367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.

Antibodies

DHPS Antibody (PACO06427)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)