Metabolism Antibodies 2
Anti-DHODH Antibody (CAB6899)
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- SKU:
- CAB6899
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DHODH Antibody |
| Antibody SKU: | CAB6899 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HL-60, SW620, HepG2, Mouse heart, Mouse spleen, Mouse kidney, Rat heart, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EIGS VTPK PQEG NPRP RVFR LPED QAVI NRYG FNSH GLSV VEHR LRAR QQKQ AKLT EDGL PLGV NLGK NKTS VDAA EDYA EGVR VLGP LADY LVVN VSSP NTAG LRSL QGKA ELRR LLTK VLQE RDGL RRVH RPAV LVKI APDL TSQD KEDI ASVV KELG IDGL IVTN TTVS RPAG LQGA LRSE TGGL SGKP LRDL STQT IREM YALT QGRV PIIG VGGV SSGQ DALE KIRA GASL VQLY TALT FWGP PVVG KVKR ELEA LLKE QGFG GVTD AIGA DHRR |
| Gene ID: | 1723 |
| Uniprot: | Q02127 |
| Cellular Location: | Mitochondrion inner membrane, Single-pass membrane protein |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | DHODH, DHOdehase, POADS, URA1 |
| Background: | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. |
| UniProt Protein Function: | DHODH: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS); also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 1.3.5.2; Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 16q22 Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleoplasm Molecular Function:dihydroorotate dehydrogenase activity Biological Process: 'de novo' pyrimidine base biosynthetic process; pyrimidine nucleoside biosynthetic process Disease: Postaxial Acrofacial Dysostosis |
| NCBI Summary: | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02127 |
| NCBI GenInfo Identifier: | 56405372 |
| NCBI Gene ID: | 1723 |
| NCBI Accession: | Q02127.3 |
| UniProt Secondary Accession: | Q02127,Q6P176, A8K8C8, |
| UniProt Related Accession: | Q02127 |
| Molecular Weight: | 42,867 Da |
| NCBI Full Name: | Dihydroorotate dehydrogenase (quinone), mitochondrial |
| NCBI Synonym Full Names: | dihydroorotate dehydrogenase (quinone) |
| NCBI Official Symbol: | DHODH |
| NCBI Official Synonym Symbols: | URA1; POADS; DHOdehase |
| NCBI Protein Information: | dihydroorotate dehydrogenase (quinone), mitochondrial |
| UniProt Protein Name: | Dihydroorotate dehydrogenase (quinone), mitochondrial |
| UniProt Synonym Protein Names: | Dihydroorotate oxidase |
| UniProt Gene Name: | DHODH |
| UniProt Entry Name: | PYRD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHODH antibody (CAB6899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens). |
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