Antibody Name:	Anti-DHFR Antibody
Antibody SKU:	CAB1607
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, 22Rv1, K-562, HL-60, THP-1, Raji, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVGS LNCI VAVS QNMG IGKN GDLP WPPL RNEF RYFQ RMTT TSSV EGKQ NLVI MGKK TWFS IPEK NRPL KGRI NLVL SREL KEPP QGAH FLSR SLDD ALKL TEQP ELAN KVDM VWIV GGSS VYKE AMNH PGHL KLFV TRIM QDFE SDTF FPEI DLEK YKLL PEYP GVLS DVQE EKGI KYKF EVYE KND
Gene ID:	1719
Uniprot:	P00374
Cellular Location:
Calculated MW:	15kDa/21kDa
Observed MW:	19kDa

Synonyms:

DHFR, DHFRP1, DYR

Background:

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - folate biosynthesis Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: nucleoplasm; cytosol Molecular Function:mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; response to methotrexate; regulation of nitric-oxide synthase activity; folic acid metabolic process; mitotic cell cycle; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency
UniProt Code:	P00374
NCBI GenInfo Identifier:	118992
NCBI Gene ID:
NCBI Accession:	P00374.2
Molecular Weight:
NCBI Full Name:	Dihydrofolate reductase
UniProt Protein Name:	Dihydrofolate reductase
Protein Family:	Viral dihydrofolate reductase
UniProt Gene Name:	DHFR
UniProt Entry Name:	DYR_HUMAN

	Western blot analysis of extracts of various cell lines, using DHFR antibody (CAB1607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A-549 cells using DHFR antibody (CAB1607).

Antibodies	ELISA Kits
Anti-DHFR Antibody (CAB9299)	Human Dihydrofolate Reductase / DHFR ELISA Kit
DHFR Antibody (PACO08821)	Human Dihydrofolate reductase (DHFR) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)