Metabolism Antibodies 2

Anti-DHCR24 Antibody (CAB5402)

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SKU:
CAB5402
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-DHCR24 Antibody
Antibody SKU:CAB5402
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 227-516 of human DHCR24 (NP_055577.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HT-29, PC-12, NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 227-516 of human DHCR24 (NP_055577.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AEIR IIPA KKYV KLRF EPVR GLEA ICAK FTHE SQRQ ENHF VEGL LYSL DEAV IMTG VMTD EAEP SKLN SIGN YYKP WFFK HVEN YLKT NREG LEYI PLRH YYHR HTRS IFWE LQDI IPFG NNPI FRYL FGWM VPPK ISLL KLTQ GETL RKLY EQHH VVQD MLVP MKCL QQAL HTFQ NDIH VYPI WLCP FILP SQPG LVHP KGNE AELY IDIG AYGE PRVK HFEA RSCM RQLE KFVR SVHG FQML YADC YMNR EEFW EMFD GSLY HKLR EKLG CQDA FPEV YDKI CKAA RH
Gene ID:1718
Uniprot:Q15392
Cellular Location:Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein
Calculated MW:54kDa/60kDa
Observed MW:55kDa
Synonyms:DHCR24, DCE, Nbla03646, SELADIN1, seladin-1
Background:This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
UniProt Protein Function:Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.
UniProt Protein Details:

Protein type:Oxidoreductase; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; EC 1.3.1.72

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; cytosol; nucleus

Molecular Function:delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; FAD binding; peptide antigen binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; negative regulation of cell proliferation; amyloid precursor protein catabolic process; protein localization; apoptosis; Ras protein signal transduction; male genitalia development; response to hormone stimulus; negative regulation of caspase activity; response to oxidative stress; cell cycle arrest; cholesterol biosynthetic process; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis

NCBI Summary:This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]
UniProt Code:Q15392
NCBI GenInfo Identifier:20141421
NCBI Gene ID:1718
NCBI Accession:Q15392.2
UniProt Related Accession:Q15392
Molecular Weight:
NCBI Full Name:Delta(24)-sterol reductase
NCBI Synonym Full Names:24-dehydrocholesterol reductase
NCBI Official Symbol:DHCR24  
NCBI Official Synonym Symbols:DCE; SELADIN1; Nbla03646; seladin-1  
NCBI Protein Information:delta(24)-sterol reductase
UniProt Protein Name:Delta(24)-sterol reductase
UniProt Synonym Protein Names:24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1
Protein Family:Delta(24)-sterol reductase
UniProt Gene Name:DHCR24  
UniProt Entry Name:DHC24_HUMAN
Anti-DHCR24 Antibody (CAB5402)Western blot analysis of extracts of various cell lines, using DHCR24 antibody (CAB5402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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