Antibody Name:	Anti-DGUOK Antibody
Antibody SKU:	CAB6898
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HepG2, Mouse heart, Mouse kidney, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RLSI EGNI AVGK STFV KLLT KTYP EWHV ATEP VATW QNIQ AAGT QKAC TAQS LGNL LDMM YREP ARWS YTFQ TFSF LSRL KVQL EPFP EKLL QARK PVQI FERS VYSD RYIF AKNL FENG SLSD IEWH IYQD WHSF LLWE FASR ITLH GFIY LQAS PQVC LKRL YQRA REEE KGIE LAYL EQLH GQHE AWLI HKTT KLHF EALM NIPV LVLD VNDD FSEE VTKQ EDLM REVN TFVK NL
Gene ID:	1716
Uniprot:	Q16854
Cellular Location:	Mitochondrion
Calculated MW:	9kDa/17kDa/21kDa/27kDa/29kDa/32kDa
Observed MW:	40kDa

Synonyms:

DGUOK, MTDPS3, NCPH, PEOB4, dGK

Background:

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene.

UniProt Protein Function:	DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; EC 2.7.1.113; Mitochondrial; Kinase, other Chromosomal Location of Human Ortholog: 2p13 Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol Molecular Function:deoxyguanosine kinase activity; nucleoside kinase activity; ATP binding Biological Process: purine deoxyribonucleoside metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; dGTP metabolic process; guanosine metabolic process; protein amino acid phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process Disease: Mitochondrial Dna Depletion Syndrome 3 (hepatocerebral Type)
NCBI Summary:	In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16854
NCBI GenInfo Identifier:	23503050
NCBI Gene ID:	1716
NCBI Accession:	Q16854.2
UniProt Secondary Accession:	Q16854,P78532, Q16759, Q4ZG09, Q7L1W9, Q96BC1,
UniProt Related Accession:	Q16854
Molecular Weight:	277
NCBI Full Name:	Deoxyguanosine kinase, mitochondrial
NCBI Synonym Full Names:	deoxyguanosine kinase
NCBI Official Symbol:	DGUOK
NCBI Official Synonym Symbols:	dGK; MTDPS3
NCBI Protein Information:	deoxyguanosine kinase, mitochondrial
UniProt Protein Name:	Deoxyguanosine kinase, mitochondrial
UniProt Gene Name:	DGUOK
UniProt Entry Name:	DGUOK_HUMAN

	Western blot analysis of extracts of various cell lines, using DGUOK antibody (CAB6898) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse brain using DGUOK antibody (CAB6898) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using DGUOK antibody (CAB6898) at dilution of 1:100 (40x lens).