Name: Anti-DGKQ Antibody (CAB3825)
Brand: Cell Biology Antibodies 9
SKU: CAB3825
Availability: OutOfStock

Antibody Name:	Anti-DGKQ Antibody
Antibody SKU:	CAB3825
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 683-942 of human DGKQ (NP_001338.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, K-562, MCF-7, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 683-942 of human DGKQ (NP_001338.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GRVL RWGA GYSG EDPF SVLL SVDE ADAV LMDR WTIL LDAH EAGS AEND TADA EPPK IVQM SNYC GIGI DAEL SLDF HQAR EEEP GKFT SRLH NKGV YVRV GLQK ISHS RSLH KQIR LQVE RQEV ELPS IEGL IFIN IPSW GSGA DLWG SDSD TRFE KPRM DDGL LEVV GVTG VVHM GQVQ GGLR SGIR IAQG SYFR VTLL KATP VQVD GEPW VQAP GHMI ISAA GPKV HMLR KAKQ KPRR AGTT RDAR ADAA PAPE SDPR
Gene ID:	1609
Uniprot:	P52824
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Nucleus speckle, cytoskeleton
Calculated MW:	101kDa
Observed MW:	101kDa

Synonyms:	DGKQ, DAGK, DAGK4, DAGK7
Background:	The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction.

UniProt Protein Function:	DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.
UniProt Protein Details:	Protein type:EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, lipid Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: cytoplasm; cytoskeleton; cytosol; nuclear speck; nucleus; plasma membrane Molecular Function:diacylglycerol kinase activity; kinase binding; phospholipase binding; protein binding; transcription activator binding Biological Process: cAMP-mediated signaling; G-protein coupled receptor protein signaling pathway; platelet activation; regulation of transcription from RNA polymerase II promoter; response to ATP
NCBI Summary:	The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]
UniProt Code:	P52824
NCBI GenInfo Identifier:	257051005
NCBI Gene ID:	1609
NCBI Accession:	P52824.2
UniProt Secondary Accession:	P52824,Q6P3W4,
UniProt Related Accession:	P52824
Molecular Weight:	101,155 Da
NCBI Full Name:	Diacylglycerol kinase theta
NCBI Synonym Full Names:	diacylglycerol kinase theta
NCBI Official Symbol:	DGKQ
NCBI Official Synonym Symbols:	DAGK; DAGK4; DAGK7
NCBI Protein Information:	diacylglycerol kinase theta
UniProt Protein Name:	Diacylglycerol kinase theta
UniProt Synonym Protein Names:	Diglyceride kinase theta; DGK-theta
Protein Family:	Diacylglycerol kinase
UniProt Gene Name:	DGKQ
UniProt Entry Name:	DGKQ_HUMAN

	Western blot analysis of extracts of various cell lines, using DGKQ antibody (CAB3825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human breast cancer using DGKQ antibody (CAB3825) at dilution of 1:100 (40x lens).