Cell Biology Antibodies 11
Anti-DGKE Antibody (CAB7752)
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- SKU:
- CAB7752
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DGKE Antibody |
| Antibody SKU: | CAB7752 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, HeLa, 293T, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CSLQ RSRR QLHR RDIF RKSK HGWR DTDL FSQP TYCC VCAQ HILQ GAFC DCCG LRVD EGCL RKAD KRFQ CKEI MLKN DTKV LDAM PHHW IRGN VPLC SYCM VCKQ QCGC QPKL CDYR CIWC QKTV HDEC MKNS LKNE KCDF GEFK NLII PPSY LTSI NQMR KDKK TDYE VLAS KLGK QWTP LIIL ANSR SGTN MGEG LLGE FRIL LNPV QVFD VTKT PPIK A |
| Gene ID: | 8526 |
| Uniprot: | P52429 |
| Cellular Location: | Cytoplasm, Membrane, Multi-pass membrane protein |
| Calculated MW: | 20kDa/63kDa |
| Observed MW: | 64kDa |
| Synonyms: | DGKE, AHUS7, DAGK5, DAGK6, DGK, NPHS7 |
| Background: | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. |
| UniProt Protein Function: | DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.7.1.107; Motility/polarity/chemotaxis; Kinase, lipid Chromosomal Location of Human Ortholog: 17q22 Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane Molecular Function:metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process Disease: Nephrotic Syndrome, Type 7 |
| NCBI Summary: | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52429 |
| NCBI GenInfo Identifier: | 4503313 |
| NCBI Gene ID: | 8526 |
| NCBI Accession: | NP_003638.1 |
| UniProt Related Accession: | P52429 |
| Molecular Weight: | ~ 64kDa |
| NCBI Full Name: | diacylglycerol kinase epsilon |
| NCBI Synonym Full Names: | diacylglycerol kinase epsilon |
| NCBI Official Symbol: | DGKE |
| NCBI Official Synonym Symbols: | DGK; AHUS7; DAGK5; DAGK6; NPHS7 |
| NCBI Protein Information: | diacylglycerol kinase epsilon |
| UniProt Protein Name: | Diacylglycerol kinase epsilon |
| UniProt Synonym Protein Names: | Diglyceride kinase epsilon |
| Protein Family: | Deoxyguanosine kinase |
| UniProt Gene Name: | DGKE |
| UniProt Entry Name: | DGKE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DGKE antibody (CAB7752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody (CAB7752) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody (CAB7752) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using DGKE antibody (CAB7752) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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