Cell Biology Antibodies 5
Anti-DGKD Antibody (CAB15115)
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- SKU:
- CAB15115
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DGKD Antibody |
| Antibody SKU: | CAB15115 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 960-1140 of human DGKD (NP_690618.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Raji, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 960-1140 of human DGKD (NP_690618.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LPRP PSCS LHPE MLSE EEAT QMDQ FGQA AGVL IHSI REIA QSHR DMEQ ELAH AVNA SSKS MDRV YGKP RTTE GLNC SFVL EMVN NFRA LRSE TELL LSGK MALQ LDPP QKEQ LGSA LAEM DRQL RRLA DTPW LCQS AEPG DEES VMLD LAKR SRSG KFRL VTKF KKEK NNKN KEAH SSLG A |
| Gene ID: | 8527 |
| Uniprot: | Q16760 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 130kDa/134kDa |
| Observed MW: | 160kDa |
| Synonyms: | DGKD, DGKdelta, dgkd-2 |
| Background: | This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | DGKD: May function as signaling molecule. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, lipid; EC 2.7.1.107 Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: cytoplasm; plasma membrane Molecular Function:diacylglycerol kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity Biological Process: diacylglycerol metabolic process; platelet activation; protein homooligomerization; response to organic substance; signal transduction |
| NCBI Summary: | This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16760 |
| NCBI GenInfo Identifier: | 116241328 |
| NCBI Gene ID: | 8527 |
| NCBI Accession: | Q16760.4 |
| UniProt Secondary Accession: | Q16760,Q14158, Q6PK55, Q8NG53, |
| UniProt Related Accession: | Q16760 |
| Molecular Weight: | 130,326 Da |
| NCBI Full Name: | Diacylglycerol kinase delta |
| NCBI Synonym Full Names: | diacylglycerol kinase delta |
| NCBI Official Symbol: | DGKD |
| NCBI Official Synonym Symbols: | dgkd-2; DGKdelta |
| NCBI Protein Information: | diacylglycerol kinase delta |
| UniProt Protein Name: | Diacylglycerol kinase delta |
| UniProt Synonym Protein Names: | 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta |
| Protein Family: | Diacylglycerol kinase |
| UniProt Gene Name: | DGKD |
| UniProt Entry Name: | DGKD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DGKD antibody (CAB15115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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