Cell Biology Antibodies 17

Anti-DGCR8 Antibody (CAB18630)

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SKU:
CAB18630
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DGCR8 Antibody
Antibody SKU:CAB18630
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human DGCR8.
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, 293T, Mouse testis
Immunogen:Recombinant protein of human DGCR8.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:54487
Uniprot:Q8WYQ5
Cellular Location:Nucleus, nucleolus
Calculated MW:32kDa/82kDa/86kDa
Observed MW:110kDa
Anti-DGCR8 Antibody (CAB18630)Western blot - DGCR8 Rabbit pAb (CAB18630)
Synonyms:DGCR8, C22orf12, DGCRK6, Gy1, pasha, microprocessor complex subunit DGCR8
Background:This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:DGCR8: a double strand RNA binding protein (dsRBP) which is essential for the first processing step of primary miRNAs (pri-miRNAs). Apparently binds and is activated by heme. A nuclear complex of Drosha with DGCR8 cleaves pri-miRNA substrates into 60-70 nucleotide intermediates (pre-miRNA), thus initiating the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 (Pasha) anchors substrate pri-miRNAs. Pre-miRNAs are then processed by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs.
UniProt Protein Details:

Protein type:RNA processing; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: cytoplasm; microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function:double-stranded RNA binding; heme binding; protein binding; protein homodimerization activity; ribonuclease III activity

Biological Process: primary microRNA processing; RNA-mediated gene silencing

Disease: Velocardiofacial Syndrome

NCBI Summary:This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
UniProt Code:Q8WYQ5
NCBI GenInfo Identifier:23813990
NCBI Gene ID:54487
NCBI Accession:Q8WYQ5.1
UniProt Secondary Accession:Q8WYQ5,Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8 Q9H6T7, Q9NRW2, B2R8G1,
UniProt Related Accession:Q8WYQ5
Molecular Weight:82,750 Da
NCBI Full Name:Microprocessor complex subunit DGCR8
NCBI Synonym Full Names:DGCR8 microprocessor complex subunit
NCBI Official Symbol:DGCR8  
NCBI Official Synonym Symbols:Gy1; pasha; DGCRK6; C22orf12  
NCBI Protein Information:microprocessor complex subunit DGCR8
UniProt Protein Name:Microprocessor complex subunit DGCR8
UniProt Synonym Protein Names:DiGeorge syndrome critical region 8
Protein Family:Microprocessor complex
UniProt Gene Name:DGCR8  
UniProt Entry Name:DGCR8_HUMAN
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