Epigenetics & Nuclear Signaling Antibodies 1

Anti-DGCR14 Antibody (CAB13174)

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SKU:
CAB13174
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DGCR14 Antibody
Antibody SKU:CAB13174
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DGCR14 (NP_073210.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-549, NIH/3T3, Mouse testis, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DGCR14 (NP_073210.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:METP GASA SSLL LPAA SRPP RKRE AGEA GAAT SKQR VLDE EEYI EGLQ TVIQ RDFF PDVE KLQA QKEY LEAE ENGD LERM RQIA IKFG SALG KMSR EPPP PYVT PATF ETPE VHAG TGVV GNKP RPRG RGLE DGEA GEEE EKEP LPSL DVFL SRYT SEDN ASFQ EIME VAKE RSRA RHAW LYQA EEEF EKRQ KDNL ELPS AEHQ AIES SQAS VETW KYKA KNSL MYYP EGVP DEEQ LFKK PRQV VHKN TRFL RDPF SQAL SRCQ LQQA AALN AQHK QGKV GPDG KELI PQES PRVG GFGF
Gene ID:8220
Uniprot:Q96DF8
Cellular Location:Nucleus
Calculated MW:52kDa
Observed MW:53kDa
Synonyms:DGCR14, DGCR13, DGS-H, DGS-I, DGSH, DGSI, ES2, ESS-2, Es2el, bis1
Background:This gene is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of 22q11.2. The encoded protein may be a component of C complex spliceosomes, and the orthologous protein in the mouse localizes to the nucleus. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:DGCR14: involved in pre-mRNA splicing. One of the many proteins in the spliceosome C complex. Defects in this gene play a part in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS).
UniProt Protein Details:

Protein type:Spliceosome; RNA splicing; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 22q11.21|22q11.2

Cellular Component: nucleus

Molecular Function:protein binding

Biological Process: nervous system development; nuclear mRNA splicing, via spliceosome

Disease: Velocardiofacial Syndrome

NCBI Summary:This gene is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of 22q11.2. The encoded protein may be a component of C complex spliceosomes, and the orthologous protein in the mouse localizes to the nucleus. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
UniProt Code:Q96DF8
NCBI GenInfo Identifier:27805463
NCBI Gene ID:8220
NCBI Accession:Q96DF8.1
UniProt Secondary Accession:Q96DF8,Q49AH7, Q9BTZ4,
UniProt Related Accession:Q96DF8
Molecular Weight:52,568 Da
NCBI Full Name:Protein DGCR14
NCBI Synonym Full Names:DiGeorge syndrome critical region gene 14
NCBI Official Symbol:DGCR14  
NCBI Official Synonym Symbols:ES2; DGSH; DGSI; DGS-H; DGS-I; Es2el; DGCR13  
NCBI Protein Information:protein DGCR14
UniProt Protein Name:Protein DGCR14
UniProt Synonym Protein Names:DiGeorge syndrome critical region 13; DiGeorge syndrome critical region 14; DiGeorge syndrome protein H; DGS-H; Protein ES2
Protein Family:Protein
UniProt Gene Name:DGCR14  
UniProt Entry Name:DGC14_HUMAN
Anti-DGCR14 Antibody (CAB13174)Western blot analysis of extracts of various cell lines, using DGCR14 antibody (CAB13174) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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