Name: Anti-DFNA5/GSDME Antibody (CAB7432)
Brand: Cell Death Antibodies 2
SKU: CAB7432
Availability: OutOfStock

Antibody Name:	Anti-DFNA5/GSDME Antibody
Antibody SKU:	CAB7432
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5/GSDME (NP_004394.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, SH-SY5Y, mouse brain, rat lung, rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5/GSDME (NP_004394.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFAK ATRN FLRE VDAD GDLI AVSN LNDS DKLQ LLSL VTKK KRFW CWQR PKYQ FLSL TLGD VLIE DQFP SPVV VESD FVKY EGKF ANHV SGTL ETAL GKVK LNLG GSSR VESQ SSFG TLRK QEVD LQQL IRDS AERT INLR NPVL QQVL EGRN EVLC VLTQ KITT MQKC VISE HMQV EEKC GGIV GIQT KTVQ VSAT EDGN VTKD SNVV LEIP AATT IAYG VIEL YVKL DGQF EFCL LRGK QGGF ENKK RIDS VYLD PLVF
Gene ID:	1687
Uniprot:	O60443
Cellular Location:
Calculated MW:	10kDa/36kDa/54kDa
Observed MW:	55kDa

Synonyms:	DFNA5, ICERE-1, GSDME
Background:	Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	DFNA5: Involved in apoptosis and cell survival. Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53. Defects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5). DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Is a tumor suppressor gene with an important role in major types of tumors. Could be a valuable molecular marker in cancer. Belongs to the gasdermin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: 7p15 Cellular Component: cytoplasm Biological Process: negative regulation of cell proliferation; sensory perception of sound; inner ear receptor cell differentiation; apoptosis Disease: Deafness, Autosomal Dominant 5
NCBI Summary:	Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O60443
NCBI GenInfo Identifier:	13124046
NCBI Gene ID:	1687
NCBI Accession:	O60443.2
UniProt Secondary Accession:	O60443,O14590, Q08AQ8, Q9UBV3, A4D156, B2RAX9, B3KT05
UniProt Related Accession:	O60443
Molecular Weight:	496
NCBI Full Name:	Non-syndromic hearing impairment protein 5
NCBI Synonym Full Names:	deafness, autosomal dominant 5
NCBI Official Symbol:	DFNA5
NCBI Official Synonym Symbols:	ICERE-1
NCBI Protein Information:	non-syndromic hearing impairment protein 5; nonsyndromic hearing impairment protein; inversely correlated with estrogen receptor expression 1
UniProt Protein Name:	Non-syndromic hearing impairment protein 5
UniProt Synonym Protein Names:	Inversely correlated with estrogen receptor expression 1; ICERE-1
Protein Family:	Non-syndromic hearing impairment protein
UniProt Gene Name:	DFNA5
UniProt Entry Name:	DFNA5_HUMAN

	Western blot analysis of extracts of various cell lines, using DFNA5/GSDME antibody (CAB7432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human pulmonary tuberculosis using DFNA5/GSDME Rabbit pAb (CAB7432) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Human pancreatic panniculitis using DFNA5/GSDME Rabbit pAb (CAB7432) at dilution of 1:50 (40x lens).