Antibody Name:	Anti-DFFA Antibody [KO Validated]
Antibody SKU:	CAB1032
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, H460, HepG2, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEVT GDAG VPES GEIR TLKP CLLR RNYS REQH GVAA SCLE DLRS KACD ILAI DKSL TPVT LVLA EDGT IVDD DDYF LCLP SNTK FVAL ASNE KWAY NNSD GGTA WISQ ESFD VDET DSGA GLKW KNVA RQLK EDLS SIIL LSEE DLQM LVDA PCSD LAQE LRQS CATV QRLQ HTLQ QVLD QREE VRQS KQLL QLYL QALE KEGS LLSK QEES KAAF GEEV DAVD TGIS RETS SDVA LASH ILTA LREK QAPE LSLS SQDL ELVT KEDP KALA VALN WDIK KTET VQEA CERE LALR LQQT QSLH SLRS ISAS KASP PGDL QNPK RARQ DPT
Gene ID:	1676
Uniprot:	O00273
Cellular Location:	Cytoplasm
Calculated MW:	29kDa/36kDa
Observed MW:	35kDa, 45kDa

Synonyms:

DFFA, DFF-45, DFF1, ICAD

Background:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Hydrolase Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytosol; lipid particle; nuclear chromatin; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding Biological Process: DNA fragmentation during apoptosis; negative regulation of deoxyribonuclease activity
NCBI Summary:	Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O00273
NCBI GenInfo Identifier:	2810997
NCBI Gene ID:	1676
NCBI Accession:	O00273.1
UniProt Secondary Accession:	O00273,Q5T6G5, Q5T6G6, Q96I97, Q9Y6C6,
UniProt Related Accession:	O00273
Molecular Weight:	37kDa
NCBI Full Name:	DNA fragmentation factor subunit alpha
NCBI Synonym Full Names:	DNA fragmentation factor subunit alpha
NCBI Official Symbol:	DFFA
NCBI Official Synonym Symbols:	DFF1; ICAD; DFF-45
NCBI Protein Information:	DNA fragmentation factor subunit alpha
UniProt Protein Name:	DNA fragmentation factor subunit alpha
UniProt Synonym Protein Names:	DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD
Protein Family:	DNA fragmentation factor
UniProt Gene Name:	DFFA

	Western blot analysis of extracts of various cell lines, using DFFA antibody (CAB1032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Western blot analysis of extracts from normal (control) and DFFA knockout (KO) HeLa cells, using DFFA antibody (CAB1032) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using DFFA antibody (CAB1032). Blue: DAPI for nuclear staining.