KO Validated Antibodies 1
Anti-DFFA Antibody (CAB1032)[KO Validated]
- SKU:
- CAB1032
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
Antibody Name: | Anti-DFFA Antibody [KO Validated] |
Antibody SKU: | CAB1032 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IF |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1). |
Application: | WB IF |
Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | Jurkat, H460, HepG2, Mouse brain, Rat brain |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | MEVT GDAG VPES GEIR TLKP CLLR RNYS REQH GVAA SCLE DLRS KACD ILAI DKSL TPVT LVLA EDGT IVDD DDYF LCLP SNTK FVAL ASNE KWAY NNSD GGTA WISQ ESFD VDET DSGA GLKW KNVA RQLK EDLS SIIL LSEE DLQM LVDA PCSD LAQE LRQS CATV QRLQ HTLQ QVLD QREE VRQS KQLL QLYL QALE KEGS LLSK QEES KAAF GEEV DAVD TGIS RETS SDVA LASH ILTA LREK QAPE LSLS SQDL ELVT KEDP KALA VALN WDIK KTET VQEA CERE LALR LQQT QSLH SLRS ISAS KASP PGDL QNPK RARQ DPT |
Gene ID: | 1676 |
Uniprot: | O00273 |
Cellular Location: | Cytoplasm |
Calculated MW: | 29kDa/36kDa |
Observed MW: | 35kDa, 45kDa |
Synonyms: | DFFA, DFF-45, DFF1, ICAD |
Background: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
UniProt Protein Function: | DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Hydrolase Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytosol; lipid particle; nuclear chromatin; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding Biological Process: DNA fragmentation during apoptosis; negative regulation of deoxyribonuclease activity |
NCBI Summary: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | O00273 |
NCBI GenInfo Identifier: | 2810997 |
NCBI Gene ID: | 1676 |
NCBI Accession: | O00273.1 |
UniProt Secondary Accession: | O00273,Q5T6G5, Q5T6G6, Q96I97, Q9Y6C6, |
UniProt Related Accession: | O00273 |
Molecular Weight: | 37kDa |
NCBI Full Name: | DNA fragmentation factor subunit alpha |
NCBI Synonym Full Names: | DNA fragmentation factor subunit alpha |
NCBI Official Symbol: | DFFA |
NCBI Official Synonym Symbols: | DFF1; ICAD; DFF-45 |
NCBI Protein Information: | DNA fragmentation factor subunit alpha |
UniProt Protein Name: | DNA fragmentation factor subunit alpha |
UniProt Synonym Protein Names: | DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD |
Protein Family: | DNA fragmentation factor |
UniProt Gene Name: | DFFA |
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