Signal Transduction Antibodies 3

Anti-DET1 homolog Antibody (CAB9974)

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SKU:
CAB9974
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DET1 homolog Antibody
Antibody SKU:CAB9974
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HVST IKPR RIQN QNVI HRLE RRRI SSGK AGTH WHQV RVFH QNVF PNFT VVNV EKPP CFLR KFSP DGRY FIAF SSDQ TSLE IYEY QGCQ AAED LLQG YEGE ILSN GNDQ RSVN IRGR LFER FFVL LHIT NVAA NGEH LNRE CSLF TDDC RCVI VGSA AYLP DEPH PPFF EVYR NSES VTPN PRSP LEDY SLHI IDLH TGRL CDTR TFKC DKVV LSHN QGLY LYKN IL
Gene ID:55070
Uniprot:Q7L5Y6
Cellular Location:Nucleus
Calculated MW:63kDa/65kDa
Observed MW:55kDa
Synonyms:DET1
Background:
UniProt Protein Function:DET1: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation. Belongs to the DET1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase

Chromosomal Location of Human Ortholog: 15q26.1

UniProt Code:Q7L5Y6
NCBI GenInfo Identifier:55976471
NCBI Gene ID:55070
NCBI Accession:Q7L5Y6.2
UniProt Secondary Accession:Q7L5Y6,Q2VPC0, Q9NWD5, B3KNN6,
UniProt Related Accession:Q7L5Y6
Molecular Weight:64kDa
NCBI Full Name:DET1 homolog
NCBI Synonym Full Names:de-etiolated homolog 1 (Arabidopsis)
NCBI Official Symbol:DET1  
NCBI Protein Information:DET1 homolog
UniProt Protein Name:DET1 homolog
UniProt Synonym Protein Names:De-etiolated-1 homolog
Protein Family:DET1
UniProt Gene Name:DET1  
Anti-DET1 homolog Polyclonal Antibody (CAB9974)Western blot analysis of extracts of various cell lines, using DET1 antibody (CAB9974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
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