Name: Anti-Desmoplakin Antibody (CAB7635)
Brand: Cell Biology Antibodies 11
SKU: CAB7635
Availability: OutOfStock

Antibody Name:	Anti-Desmoplakin Antibody
Antibody SKU:	CAB7635
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Desmoplakin (NP_004406.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, Mouse heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Desmoplakin (NP_004406.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSCN GGSH PRIN TLGR MIRA ESGP DLRY EVTS GGGG TSRM YYSR RGVI TDQN SDGY CQTG TMSR HQNQ NTIQ ELLQ NCSD CLMR AELI VQPE LKYG DGIQ
Gene ID:	1832
Uniprot:	P15924
Cellular Location:	Cell junction, Cytoplasm, cytoskeleton, desmosome
Calculated MW:	260kDa/278kDa/331kDa
Observed MW:	330kDa

Synonyms:	DCWHKTA, DP, DSP
Background:	This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Desmoplakin: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. Belongs to the plakin or cytolinker family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion Chromosomal Location of Human Ortholog: 6p24 Cellular Component: desmosome; mitochondrion; basolateral plasma membrane; plasma membrane; intermediate filament; cornified envelope; fascia adherens; nucleus Molecular Function:protein binding, bridging; protein binding; protein kinase C binding; structural constituent of cytoskeleton; cell adhesion molecule binding; structural molecule activity Biological Process: keratinocyte differentiation; cell-cell adhesion; epidermis development; wound healing; apoptosis; intermediate filament organization; peptide cross-linking; cell structure disassembly during apoptosis Disease: Cardiomyopathy, Dilated, With Woolly Hair And Keratoderma; Arrhythmogenic Right Ventricular Dysplasia, Familial, 8; Keratosis Palmoplantaris Striata Ii; Cardiomyopathy, Dilated, With Woolly Hair, Keratoderma, And Tooth Agenesis; Skin Fragility-woolly Hair Syndrome; Epidermolysis Bullosa, Lethal Acantholytic
NCBI Summary:	Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008]
UniProt Code:	P15924
NCBI GenInfo Identifier:	58530840
NCBI Gene ID:	1832
NCBI Accession:	NP_004406.2
UniProt Secondary Accession:	P15924,O75993, Q14189, Q9UHN4, B2RTT2, D7RX09,
UniProt Related Accession:	P15924
Molecular Weight:	278,916 Da
NCBI Full Name:	desmoplakin isoform I
NCBI Synonym Full Names:	desmoplakin
NCBI Official Symbol:	DSP
NCBI Official Synonym Symbols:	DP; DPI; DPII; DCWHKTA
NCBI Protein Information:	desmoplakin
UniProt Protein Name:	Desmoplakin
UniProt Synonym Protein Names:	250/210 kDa paraneoplastic pemphigus antigen
Protein Family:	Desmoplakin
UniProt Gene Name:	DSP
UniProt Entry Name:	DESP_HUMAN

	Western blot analysis of extracts of various cell lines, using Desmoplakin antibody (CAB7635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human tonsil using Desmoplakin antibody (CAB7635) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using Desmoplakin antibody (CAB7635) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human breast cancer using Desmoplakin antibody (CAB7635) at dilution of 1:100 (40x lens).